摘要
目的:对帕金森病(PD)转基因模型小鼠脑内多巴胺和大脑病理样本进行检测,探讨拜颤停复方对PD模型脑功能异常的治疗作用及神经保护作用机制。方法:运用爬杆和自主活动实验分析转基因模型小鼠行为学改变,UPLC-MS串联三重四级杆方法检测脑内多巴胺含量,TUNEL法检测大脑黑质区病理变化,运用方差分析进行组间比较。结果:与正常对照组比较,模型对照组爬杆时间显著增加(P<0.05),自主活动次数显著减少(P<0.05),多巴胺含量显著降低(P<0.05),细胞呈典型的凋亡形态学改变,如细胞变小、变圆、核固缩,细胞凋亡率显著升高(P<0.05);与模型对照组比较,拜颤停复方95.8 mg/kg组多巴胺含量明显升高(P<0.05);拜颤停复方181.5 mg/kg组爬杆时间明显减少(P<0.05),多巴胺含量明显升高(P<0.05),细胞凋亡率明显下降(P<0.05);拜颤停复方363 mg/kg组爬杆时间明显减少(P<0.05),自主活动次数明显增加(P<0.05),多巴胺含量明显升高(P<0.05),细胞凋亡率明显下降(P<0.05)。结论:拜颤停复方通过改善转基因PD模型小鼠脑功能,如减少爬杆时间、增加自主活动次数和脑内多巴胺含量,同时改善脑细胞病理变化治疗PD。
Objective: The dopamine and brain pathological samples in the brain of Parkinson’s disease(PD) transgenic model mice were tested, and the mechanism of neuroprotective effect of Baichanting compound on brain dysfunction in PD model was investigated. Methods: The behavioral changes of transgenic mice were analyzed by the climbing pole and autonomic activity tests,the dopamine content in the brain was detected by UPLC-MS series triple four pole method,the pathological changes in substantia nigra of brain were detected by TUNEL method, and the difference between groups was analyzed by variance analysis. Results: Compared with the control group,the pole climbing time significantly increased in the model group(P<0.05), the number of autonomous activities was significantly decreased(P<0.05), the dopamine content was significantly decreased(P<0.05), and the cells showed typical morphological changes of apoptosis, such as smaller cells, rounder cells and nuclear shrinkage, the rate of apoptosis was significantly increased(P<0.05). Compared with the model group, the content of dopamine in 95.75 mg/kg Baichanting compound group was significantly increased(P<0.05). In 181.5 mg/kg Baichanting compound group, the pole climbing time was significantly reduced(P<0.05),the dopamine content was significantly increased(P<0.05), and the apoptosis rate was significantly decreased(P<0.05). In 363 mg/kg Baichanting compound group, the climbing time was significantly reduced(P<0.05), the number of autonomous activities was significantly increased(P<0.05), the dopamine content was significantly increased(P<0.05), and the apoptosis rate was significantly decreased(P<0.05). Conclusion: Baichanting compound can improve the brain function of transgenic PD model mice, such as reducing climbing time, increasing the number of autonomous activities and dopamine content in the brain, and improving the pathological changes of brain cells.
作者
高鑫
刘京京
吴娟
陈平平
刘树民
Gao Xin;Liu Jingjing;Wu Juan;Chen Pingping;Liu Shumin(Research Institute of Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040;Center for Drug Safety Evaluation,Heilongjiang University of Chinese Medicine,Harbin 150040)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2020年第4期159-162,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金项目(编号:81774188)。
关键词
拜颤停
帕金森病
多巴胺
病理变化
细胞凋亡
Baichanting
Parkinson disease
Dopamine
pathological changes
cell apoptosis
