摘要
本文采用H2O2诱导的细胞氧化应激模型和脂多糖(LPS)诱导的细胞炎症模型对鲜龙牙百合80%乙醇溶液提取物及其各萃取部位进行抗氧化、抗炎活性评价,并基于超高压液相色谱串联四极杆飞行时间质谱(UPLC-Q-TOF-MS)对具有显著活性的萃取部位进行化学成分分析。结果表明,水饱和正丁醇萃取部位(50μg/mL)的抗氧化能力最强,可对RIN细胞中抗氧化酶活性分别提高2.78倍(SOD)、3.79倍(GSH)、6.27倍(CAT);三氯甲烷萃取部位(50μg/mL)的抗炎活性最强,对RAW 264.7细胞内NO、TNF-α、IL-6等炎症因子的抑制率分别达55.21%、20.86%和84.95%。其中,水饱和正丁醇部位主要由12种化合物(包括1个木脂素类化合物、5个苯丙素酸和6个甾体皂苷)组成,三氯甲烷部位主要由14种化合物(包括7个苯丙素酸、1个薯蓣皂苷元和6个甾体皂苷)组成,有4个化合物为共有组分。因此,鲜龙牙百合具抗氧化、抗炎活性的物质基础可能是苯丙素酸和甾体皂苷类化合物,且二者多集中在水饱和正丁醇和三氯甲烷萃取部位。本文旨在为研究百合具生物活性的物质基础及开发利用提供理论参考。
In this paper,the oxidative stress model induced by H2O2 and the inflammatory model induced by lipopolysaccharide(LPS)were used to evaluate the antioxidant and anti-inflammatory activities of the extracts from 80%ethanol solution of fresh Longya Lilium,and the chemical components of the extraction parts with significant activities were analyzed by ultra-high pressure liquid chromatography tandem quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS).The results showed that the water saturated n-butanol extract part(50μg/mL)had the strongest antioxidant capacity,which increased the activity of antioxidant enzymes in RIN cells by 2.78 times(SOD),3.79 times(GSH),6.27 times(CAT);the chloroform extract part(50μg/mL)had the strongest anti-inflammatory activity,and the inhibition rates of NO,TNF-α,IL-6 in RAW 264.7 cells were 55.21%,20.86%and 84.95%respectively.In addition,the antioxidant activity of water saturated n-butanol extraction part was stronger than that of chloroform,and the anti-inflammatory activity of chloroform extraction part was stronger than that of water saturated n-butanol.The water saturated n-butanol part is mainly composed of 12 compounds(including 1 lignan,5 phenylpropanoic acids and 6 steroidal saponins),the chloroform part is mainly composed of 14 compounds(including 7 phenylpropanoic acids,1 diosgenin and 6 steroidal saponins),and 4 compounds are common components.Therefore,phenylpropanoic acid and steroidal saponins may be the material basis of fresh Longya Lilium's antioxidant and anti-inflammatory activities,and most of them are concentrated in the extraction parts of water saturated n-butanol and chloroform.These results provide theoretical reference for further elucidating the material basis of Lilium's biological activities and its development and utilization.
作者
赵康宏
周峰
严思恩
刘东波
谢红旗
ZHAO Kang-hong;ZHOU Feng;YAN Si-en;LIU Dong-bo;XIE Hong-qi(College of Horticulture,Hunan Agricultural University,Changsha 410128,China;State Key Laboratory of Subhealth Intervention Technology,Changsha,410128,China;Hunan Key Laboratory of Chinese Veterinary Medicine,Changsha 410128,China)
出处
《现代食品科技》
EI
CAS
北大核心
2020年第11期87-97,共11页
Modern Food Science and Technology
基金
中央引导地方科技发展专项资金(2019XF5074)
湖南省现代农业产业技术体系建设专项(湘财农指[2019]0047号)
现代农业产业技术体系建设专项资金(CARS-21)
湖南省科技重大专项(2017SK1020)。
关键词
百合
萃取部位
抗氧化
抗炎
化学成分
Lilium
extraction part
antioxidant
anti-inflammatory
chemical components
