摘要
目的探索死后浸没大鼠肝脏中氨含量动力学变化。方法取健康SD大鼠分为两组,颈椎脱位处死后分别浸没于河水和装有25℃室温河水的水槽中,于不同时间取材并利用气相色谱-质谱法测定含量。结果对比不同有机溶剂萃取效果,乙酸乙酯:正己烷(1:1)萃取效果最好;两组大鼠肝脏中氨含量均随死亡时间延长而增加;氨的增加速率均呈现先快后慢再变快的趋势;河水浸没组大鼠肝中氨的增长速率大于室温浸没组。结论死后浸没大鼠肝脏中氨的含量变化与PMSI具有相关性,有望为晚期PMSI推断提供参考。
Objective To explore the kinetic changes of ammonia content in postmortem submerged rat liver.Methods Healthy SD rats were divided into two groups.After executing by cervical dislocation,they were immersed in summer riverand a water tank containing 25°C room temperature river water respectively,and the liver tissues were taken at different postmortem interval periods and ammonia content was determined by gas chromatography-mass spectrometry(GC-MS).Results Compared with the extraction of different organic solvents,ethyl acetate:n-hexane(1:1)has the best extraction effect.The content of ammonia in the liver of both groups increased with the prolongation of postmortem interval,The increasing rate of ammonia shows the trend of fast,slow and then fast.Overall,the growth rate of ammonia in the liver of rats immersed in summer river was faster than that in the group immersed in room temperature.Conclusion The changes of ammonia content in rat liver after death is correlated with PMSI,whcih is expected to provide reference for late PMSI inference.
作者
夏玮
袁慧雅
高利娜
徐恩宇
刘俊亭
Xia Wei;Yuan Huiya;Gao Lina;Xu Enyu;Liu Junting(Department of Toxicology Analysis,School of Forensic Medicine,China Medical University Shengyang 110001,Liaoning)
出处
《中国法医学杂志》
CSCD
2020年第5期466-470,474,共6页
Chinese Journal of Forensic Medicine
基金
国家自然科学基金面上项目(81671864)。
关键词
法医病理学
死后浸没时间
氨
气质联用(GC-MS)
Forensic pathology
Postmortem immersion time
Ammonia
Gas chromatography-mass spectrometry(GC-MS)
