摘要
为检测黄瓜籽粉中的有效成分β-谷甾醇,文章采用均匀设计法优化黄瓜籽粉β-谷甾醇的提取工艺,滤纸片法探究黄瓜籽粉中β-谷甾醇提取物对肺炎双球菌、金黄色葡萄球菌和流感嗜血杆菌的体外抑菌作用,通过体外抗氧化实验探讨黄瓜籽粉中β-谷甾醇的抗氧化作用.研究结果表明:四因素五水平均匀设计下料液比1:6、碱浓度10%、皂化温度70℃、皂化时间30 min为最优组合;不同浓度的黄瓜籽粉β-谷甾醇提取物对金黄色葡萄球菌、流感嗜血杆菌、肺炎双球菌的抑制效果差异显著;黄瓜籽粉中的β-谷甾醇具有一定的抗氧化活性,并且清除能力与β-谷甾醇浓度成正比.
To detect the active componentsβ-sitosterols of cucumber seed powder,the extraction process of cucumber seed powderβ-sitosterol was optimized by uniform design method,and the in vitro bacteriostatic effect of glutsterol extract from cucumber seed powder on pneumococcus,Staphylococcus aureus and Haemophilus influenzae was investigated by filter paper method.The antioxidant effect ofβ-sitosterols in cucumber seed powder was studied by in vitro antioxidant test.The results showed that the optimal combination was the ratio of feed stock to liquid of 1:6,alkali concentration of 10%,saponification temperature of 70℃and saponification time of 40 min.Different concentrations of cucumber seed powderβ-glutsterol extract on Staphylococcus aureus,Haemophilus influenzae,pneumococcal inhibition effect is significantly different.Theβ-sitosterols in cucumber seed powder have certain antioxidant activity,and the scavenging ability is proportional to the concentration ofβ-sitosterol.
作者
陈凤清
孙秋芹
傅玉全
孙士红
李童
张慧男
丛建民
CHEN Feng-qing;SUN Qiu-qin;FU Yu-quan;SUN Shi-hong;LI Tong;ZHANG Hui-nan;CONG Jian-min(College of Life Sciences,Baicheng Normal University;Department of Basic Medicine,Baicheng Medical College,Baicheng 137000,China)
出处
《白城师范学院学报》
2020年第5期27-32,69,共7页
Journal of Baicheng Normal University
基金
白城师范学院大学生创新创业训练计划项目“黄瓜籽粉止咳祛痰活性成分研究”(201910206119).
关键词
黄瓜籽粉
Β-谷甾醇
均匀设计
抑菌
抗氧化
cucumber seed powder
β-sitosterol
uniform design
bacteriostasis
antioxidation
