摘要
为了弥补溶藻液体菌剂稳定性低、不易运输以及保存时间短等缺陷,利用本研究从太湖土著花锢鱼内脏中筛选出的1株编号为GHJ的具有高效溶藻的微杆菌(Microbacterium oleivorans SP.),以小麦粉作为载体,蔗糖、谷氨酸钠、脱脂乳粉、甘油作低温保护剂,采用真空冷冻干燥法制备溶藻菌GHJ干粉菌剂,借助响应面法优化不同保护剂组成条件下菌剂的制备参数,考察了各因素及其交互作用对干粉菌剂活菌率的影响,并对冷冻干燥的干粉菌剂中保护剂不同成份对菌体保护作用的机理进行分析。结果表明,该模型预测的溶藻菌GHJ干粉菌剂的活菌率最高为189.26%,最佳复合保护剂配比为10%蔗糖∶10%甘油∶4%脱脂乳粉∶8%谷氨酸钠=1∶1∶0∶1,其机制是通过甘油维持细胞结构,蔗糖降低细胞机械损伤程度,谷氨酸钠修复受损细胞,三者共同作用实现对菌体的保护。本研究对于溶藻菌干粉菌剂制备及商品化开发有一定的理论和应用参考价值。
In order to make up the defects such as low stability,poor transportation,and short preservation time of algicidal bacteria liquid agent,a strain of Microbacterium oleivorans SP.(GHJ)with high-efficiency algicidal rate was isolated from the viscera of indigenous fish in Taihu Lake by our research group.With wheat flour as the carrier,sucrose,sodium glutamate,skim milk powder and glycerol as the protective agents,algicidal bacteria GHJ dry powder was prepared by vacuum freeze-drying method.With the aid of response suface method(RSM),the preparation parameters of different protective agents were optimized,and the effects of various factors and their interactions on the living bacteria rate of dry powder agents was investigated.Besides,the mechanism of different components of protective agents in the freeze-drying powder to protect bacteria was also analyzed.The results showed that the living bacteria rate of GHJ dry powder agent predicted by this model was 189.26%,and the best compound protective agent ratio was 10%sucrose∶10%glycerol∶4%skim milk powder∶8%sodium glutamate=1∶1∶0∶1.The mechanism was that glycerol maintained cell structure,sucrose reduced the degree of cellular mechanical damage,and sodium glutamate repaired the damaged cells.The three work together to achieve the protection of bacteria.This study has certain theoretical and practical reference value for the preparation and commercial development of algicidal dry powder agents.
作者
周健飞
申晓霞
徐瑾
郭惠娟
王美娟
张文艺
ZHOU Jianfei;SHEN Xiaoxia;XU Jin;GUO Huijuan;WANG Meijuan;ZHANG Wenyi(School of Environmental&Safety Engineering,Changzhou University,Changzhou,Jiangsu 213164,China;Jiangsu Longhuan Environmental Technology Co.,Ltd.,Changzhou,Jiangsu 213164,China)
出处
《天津农业科学》
CAS
2020年第9期5-10,共6页
Tianjin Agricultural Sciences
基金
国家自然科学基金(41571471)
水体污染控制与治理科技重大专项(2017ZX07202-003/004)。
关键词
真空冷冻干燥
干粉菌剂
保护剂
响应面
活菌率
vacuum freeze-drying
dry powder agent
protective agents
response surface methodology
living bacteria rate
