摘要
为探究GLIS1基因表达水平与绵羊产羔数之间的关系,利用半定量RT-PCR(Semi-quantitative RT-PCR,sqRT-PCR)和实时荧光定量PCR(Quantitative real-time PCR,qPCR)技术检测了绵羊GLIS1基因在不同产羔能力绵羊各组织中的表达情况。结果表明:GLIS1基因在多羔小尾寒羊输卵管高表达,在单羔苏尼特羊卵巢、输卵管、子宫体以及子宫角中的表达量均高于多羔小尾寒羊(P>0.05);GLIS1基因B+基因型在各组织中的表达量均极显著高于BB基因型和++基因型(P<0.01)。综上得出,GLIS1基因可能通过调控其mRNA表达水平对绵羊产羔数发挥负调控作用。
To investigate the association between expression level and litter size in sheep,semi-quantitative RT-PCR(sqRT-PCR)and quantitative real-time PCR(qPCR)were performed to detect the expression of GLIS1 gene in sheep with different lambing capacity.The results showed that the expression of GLIS1 was at high level in the oviduct of Small Tail Han sheep,the expression in ovary,oviduct,uterus body and uterine horn of monotocous Sunite sheep was higher than that in polytocous Small Tail Han sheep;the expression of GLIS1 in B+was extremely significant higher than that in BB and++(P<0.01).Therefore,it can be concluded that GLIS1 gene may play a negative regulatory role in the litter size of sheep by regulating its mRNA expression level.
作者
周梅
狄冉
胡文萍
王翔宇
王重龙
张效生
张金龙
刘秋月
储明星
Zhou Mei;Di Ran;Hu Wenping;Wang Xiangyu;Wang Chonglong;Zhang Xiaosheng;Zhang Jinlong;Liu Qiuyue;Chu Mingxing(Key Laboratory of Animal Genetics,Breeding and Reproduction of Ministry of Agriculture and Rural Affairs,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Key Laboratory of Pig Molecular Quantitative Genetics of Anhui Academy of Agricultural Sciences,Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering,Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences,Hefei 230031,China;Tianjin Institute of Animal Sciences,Tianjin 300381,China)
出处
《中国草食动物科学》
CAS
2020年第6期6-9,31,共5页
China Herbivore Science
基金
国家转基因科技重大专项(2016ZX08009-003-006和2016ZX08010-005-003)
国家自然科学基金项目(31772580)
国家肉羊产业技术体系专项(CARS-38)
中国农业科学院科技创新工程(ASTIP-IAS13)
农业科研杰出人才及其创新团队项目(农办人[2015]62号)
国家万人计划科技创新领军人才项目(W02020274)
天津市农业科技成果转化与推广项目(201704020)
博士启动基金。
