摘要
Membrane-associated guanylate kinase(MAGUK)family protein MAGUK invert 2(MAGI-2)has been demonstrated to be involved in the tumorigenic mechanism of prostate cancer.The objective of this study was to investigate the expression of MAGI・2 at mRNA and protein levels.The prog no stic value of MAGI-2 in Han Chin ese patie nts with prostate cancer was also investigated.The expression data of MAGI・2 were assessed through database retrieval,analysis of sequencing data from our group,and tissue immunohistochemistry using digital scoring system(H・score).The clinical,pathological,and follow-up data were collected.The expression of MAGI-2 in prostate tumor tissues and prostate normal tissues was evaluated and compared.MAGI-2 expression was associated with clinical parameters including tumor stage,lymph node status,Gleason score,PSA level,and biochemical recurrenee of prostate cancer.The relative expression of MAGI-2 mRNA was lower in the tumor tissue in The Cancer Genome Atlas(TCGA)database and sequencing data(P<0.001).There was no difference in MAGI-2 protein expression between tumor and normal tissues in tissue microarray(TMA)results.MAGI-2 expression was associated with pathological tumor stage(P=0.02),Gleason score(P=0.05),and preoperation prostate-specific antigen(PSA;P=0.04).A positive correlation was identified between MAGI-2 and phosphatase and tensin homolog deleted on chromosome 10(PTEN)expressions through the analysis of TCGA and TMA data(P<0.0001).Patients with higher MAGI-2 expression had longer biochemical recurrence-free survival in the univariate analysis(P=0.005),which in dicates an optimal prog no stic value of MAGI-2 in Han Chin ese patie nts with prostate can cer.I n con elusion,MAGI-2 expressi on gradually decreases with tumor progression,and can be used as a predictor of tumor recurrence in Chinese patients.
基金
The authors acknowledge the contribution of Dr.Lin Zhao,Dr.Ya-Sheng Zhu,Dr.Zhe-Xu Cao,and Dr.Chen Ye from the Department of Urology,Changhai Hospital,Naval Medical University(the Second Military Medical University),for their kind help during the experimental process.This study was supported by the National Natural Science Foundation of China(No.81430058)
Shanghai Key Laboratory of Cell Engineering(No.14DZ2272300).
