摘要
目的:研究滇黄芩醇提物及不同溶剂萃取部位对四氯化碳(CCl4)致小鼠肝损伤的保护作用。方法:用95%乙醇提取滇黄芩得到醇提物浸膏;再经乙酸乙酯、正丁醇依次萃取,得不同溶剂萃取部位。从48只小鼠中随机选取8只为正常组,其余40只为造模组。正常组小鼠腹腔注射给予等体积橄榄油,每3日1次,连续6周;造模组小鼠腹腔注射给予30%CCl4橄榄油溶液,首次剂量为5 mL/kg,以后每次3 m L/kg,每3日1次,连续6周,造成肝损伤模型。将造模成功后的小鼠随机分为模型组(生理盐水)、水飞蓟宾组(阳性对照,20 mg/kg)、滇黄芩醇提物组(100 mg/kg)、滇黄芩乙酸乙酯组(100 mg/kg)、滇黄芩正丁醇组(100 mg/kg),每组8只。每日灌胃1次,连续给药6周。观察各组小鼠实验期间的一般状态;末次给药1 h后,采用酶标仪检测其血清中总胆固醇(TC)、三酰甘油(TG)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)的含量;经苏木精-伊红染色后,观察其肝组织病理形态学变化并进行Ishak评分。结果:正常组小鼠活动正常,毛发浓密有光泽,体质量正常增长,肝组织未见明显病理形态学改变;模型组小鼠精神萎糜,毛发蓬乱无光泽,体质量(给药前及给药第1、2周)均较正常组显著减轻(P<0.05或P<0.01);与正常组比较,模型组小鼠血清中TC、TG、ALT和AST含量均显著升高(P<0.01或P<0.05);肝小叶结构受到破坏严重,炎症细胞浸润严重,肝索组织排列紊乱,Ishak评分显著升高(P<0.01)。与模型组比较,各给药组小鼠上述症状及肝组织病理损伤均有不同程度的改善;水飞蓟宾组、滇黄芩乙酸乙酯组小鼠血清中TC、ALT、AST含量,各给药组小鼠血清中TG含量以及水飞蓟宾组、滇黄芩醇提物组、滇黄芩乙酸乙酯组小鼠肝组织Ishak评分均显著降低(P<0.05或P<0.01)。结论:滇黄芩醇提物及其不同溶剂萃取部位对CCl4致肝损伤模型小鼠均有不同程度的肝保护作用,乙酸乙酯部位可能是其护肝活性部位。
OBJECTIVE:To study the protective effects of Scutellaria amoena enthanol extract and its different solvent parts on liver injury induced by CCl4.METHODS:S.amoena was extracted with 95%ethanol to obtain ethanol extract,and then was respectively extracted with ethyl acetate and n-butanol to obtain corresponding polar parts.Totally 48 mice were randomly divided into normal group(8 mice)and modeling group(40 mice).Normal group was given constant volume of olive oil intraperitoneally,3 times a day,for consecutive 6 weeks.Model group was given 30%CCl4-olive oil solution intraperitoneally to induce liver injury model,with initial dose of 5 mL/kg after each 3 mL/kg,3 times a days,for 6 consecutive weeks.After modeling,the mice were randomly divided into model group(normal saline),sylibin group(positive control,20 mg/kg),S.amoena ethanol extract group(100 mg/kg),S.amoena ethyl acetate group(100 mg/kg),and S.amoena n-butanol group(100 mg/kg),with 8 mice in each group.After they were given relevant medicine intragastrically once a day,for consecutive 6 weeks.The general information during experiment of mice was observed.1 h after last medication,the serum contents of TC,TG,ALT and AST were determined by Enzyme-labelled meter.After HE staining,the pathological changes of liver tissue were observed and Ishak score was performed.RESULTS:In normal group,mice had normal activity,thick and glossy hair,and the body weight was increased.The liver tissue had no obvious pathological changes.The model group had sparse hair,and they were emaciated and listlessness;and body weight(before medication,1,2 week after medication)was significantly lower than normal group(P<0.05 or P<0.01).Compared with normal group,the contents of TC,TG,ALT and AST in serum were increased significantly(P<0.01 or P<0.05).The structure of hepatic lobule was severely damaged and had more inflammatory cell infiltration;the arrangement of hepatic cord was disordered and the Ishak score was significantly increased(P<0.001).Compared with model group,above symptom and liver injury of mice in different administration groups were improved to different extents.The serum contents of TC,ALT and AST in silybin group and S.amoena ethyl acetate group,serum contents of TG in administration groups as well as Ishak scores of liver tissue were decreased significantly in silybin group,S.amoena ethanol extract group and S.amoena ethyl acetate group(P<0.05 or P<0.001).CONCLUSIONS:S.amoena ethanol extract and its different solvent parts can protect liver tissue of CCl4-induced liver injury model mice,and active part is the ethyl acetate part of S.amoena.
作者
付胜男
李欣坪
虎春艳
薛咏梅
林玉萍
FU Shengnan;LI Xinping;HU Chunyan;XUE Yongmei;LIN Yuping(School of Chinese Materia Medica,Yunnan University of TCM,Kunming 650500,China)
出处
《中国药房》
CAS
北大核心
2020年第22期2731-2735,共5页
China Pharmacy
基金
云南省科技计划项目中医联合面上项目[No.2017-FF117(-022)]。
关键词
滇黄芩
醇提物
萃取部位
四氯化碳
肝损伤
小鼠
Scutellaria amoena
Ethanol extract
Solvent parts
CCl4
Liver injury
Mice
