摘要
以油茶饼粕为原料,采用乙醇提取-丙酮沉淀法对茶皂素进行提取分离。以茶皂素纯度和得率为考察指标,对乙醇体积分数、液料比、提取温度、提取时间、提取次数、提取液浓缩程度和丙酮用量等工艺参数进行了单因素优化。结果表明:体积分数95%的乙醇为提取溶剂,乙醇与预处理过的油茶饼粕液料比为9∶1(mL∶g),提取温度为70℃,提取时间为4 h,提取次数为2次,提取液浓缩至刚好有固体析出,丙酮用量为4倍浓缩液体积量时提取分离效果较佳,得到的茶皂素纯度为85.17%,得率为9.82%。不同溶剂打浆对产品纯化效果的比较发现:丙酮、乙酸乙酯、无水乙醇、体积分数95%的乙醇作为打浆纯化溶剂用于提高茶皂素纯度效果均不明显。
Tea saponin was extracted and separated from Camellia oleifera seed cake by ethanol extraction and acetone precipitation process.Using the purity and yield of tea saponin as the indexes,the effects of ethanol volume fraction,liquid to solid ratio,extraction temperature,extraction time,extraction times,concentration degree of the extract,acetone amount and reslurrying solvent on extraction and purification technology were evaluated by single factor experiment.The optimum conditions were as follows:95%volume fraction of ethanol for extraction solvent,ratio of liquid to solid 9∶1(mL∶g),extraction temperature 70℃,extraction time 4 h,extraction times 2 times,the extraction solution was concentrated to just have solid precipitation,and the volume ratio of concentrated liquid to acetone was 1∶4.Under these conditions,the purity and yield of tea saponin could reach up to 85.17%and 9.82%respectively.The effects of acetone,ethyl acetate,anhydrous ethanol and 95%ethanol as reslurrying solvent on improving the purity of tea saponin were not obvious.
作者
张耀洲
谷清义
陈琼
ZHANG Yaozhou;GU Qingyi;CHEN Qiong(Biology of Pharmaceutical Engineering College,Xinyang Agriculture and Forestry University,Xinyang 464000,China)
出处
《生物质化学工程》
CAS
北大核心
2020年第6期39-44,共6页
Biomass Chemical Engineering
基金
2018年校级科技创新团队建设项目(CXTD-201805)。
关键词
油茶饼粕
茶皂素
提取
沉淀
95%乙醇
丙酮
Camellia oleifera seed cake
tea saponin
extraction
precipitation
95%ethanol
acetone
