摘要
课题组前期从健康杜仲叶片中分离得到1株内生细菌枯草芽孢杆菌DZSG23,此菌可以较好地防控小麦赤霉病。为明确芽孢杆菌DZSG23在小麦中的定殖与强化抗病机制研究,利用抗生素标记法分析了DZSG23菌体在小麦组织中的定殖情况,采用荧光定量PCR技术检测不同生育期小麦穗部PR-1、AOS、ACOI等基因的表达水平变化。结果表明,DZSG23可在苗期小麦植株和小麦穗表面稳定定殖;DZSG23浇灌接种苗期小麦后的第34天,DZSG23在苗期小麦植株的根、茎和叶中的定殖量分别达到1.437×10^6 CFU·g^-1、3.285×10^3 CFU·g^-1和2.377×10^3 CFU·g^-1;DZSG23喷施小麦穗表面15 d后,穗表面菌群密度仍可达7.146×10^3 CFU·g^-1。此外,诱导系统抗性(ISR)信号通路研究表明,拮抗菌DZSG23可以诱导PR-1和AOS基因的上调表达,表明拮抗菌DZSG23引入小麦后可诱导小麦的水杨酸(SA)和茉莉酸(JA)信号通路,增强其抗病性。
In our previous work,a strain of endophytic bacteria Bacillus subtilis DZSG23 was isolated from the leaves of Eucommia ulmoides Oliv,which could effectively control the wheat scab.This study was carried out to explicit the resistance mechanism of endophytic bacteria DZSG23 against wheat scab.The colonization property of DZSG23 in wheat was analysed by antibiotic label method,then the expression of PR-1,AOS,ACOI,ERF and SAM genes in wheat treated with DZSG23 at different growth stages were detected by quantitative realtime PCR.The results indicated that DZSG23 could successfully colonize wheat plant at seedling stage and surface of the spike.At the 34 d after inoculation with DZSG23 by pouring,the colonization of DZSG23 in roots,stems and leaves of wheat plants at seedling stage reached 1.437×10^6 CFU·g^-1,3.285×10^3 CFU·g^-1 and 2.377×10^3 CFU·g^-1,respectively.After 15 days of inoculation with DZSG23 strain by spraying on the surface of spikes,the surface microbial density of spike tissue still reached 7.146×10^3 CFU·g^-1.Expression of defense response genes PR-1 and AOS in wheat spike treated with DZSG23 were significantly higher than those of normal control groups at different growth stages,showing DZSG23 could induce systemic response through SA-and JA-dependent signaling pathways and enhance its disease resistance.
作者
徐莉
陈小洁
曹静婷
刘楚楚
丁婷
江腾
XU Li;CHEN Xiaojie;CAO Jingting;LIU Chuchu;DING Ting;JIANG Teng(School of Life Sciences,Anhui Agricultural University,Hefei 230036,China;School of Plant Protection,Anhui Agricultural University,Hefei 230036,China;Agricultural Park Management Center,Anhui Agricultural University,Hefei 230036,China)
出处
《浙江农业学报》
CSCD
北大核心
2020年第11期2001-2008,共8页
Acta Agriculturae Zhejiangensis
基金
国家重点研发计划子课题(2017YFD0201106-09)
国家级大学生创新创业训练计划(芽孢杆菌属微生物DZSG23在小麦中的定殖及强化抗病机制)。
关键词
芽孢杆菌DZSG23
小麦
定殖
抗病机制
Bacillus subtilis DZSG23
wheat
colonization
mechanism of disease resistance
