摘要
目的探讨利用高内涵筛选(HCS)分析评价重楼皂苷Ⅰ(PPⅠ)对肝癌细胞株Huh7、HepG2细胞增殖的影响。方法以肝癌细胞株Huh7、HepG2细胞为研究对象,采用HCS技术分析细胞增殖相关参数,包括细胞形态、细胞迁移速度、细胞数量、细胞面积和细胞圆度等。结果不同浓度PPⅠ处理肝癌细胞株Huh7、HepG2细胞后,细胞皱缩且轮廓变得不明显。随着PPⅠ浓度增加,Huh7、HepG2细胞迁移速度、细胞数量增长速度逐渐变慢,且其细胞面积和细胞圆度值逐渐变小。结论PPⅠ能够抑制Huh7、HepG2细胞增殖和迁移。
Objective To investigate the effect of Polyphyllin I(PPⅠ)on the proliferation of hepatoma cell lines Huh7 and HepG2 by high content screening(HCS)analysis.Methods Huh7 and HepG2 cells were used as the research objects,and HCS was used to analyze cell proliferation parameters,including cell morphology,cell migration rate,cell number,cell area and cell roundness.Results Huh7 and HepG2 cells were treated with different concentrations of PPⅠ,and the cells shrunk and the outline became indistinct.With the increase of PPⅠconcentration,the migration rate and cell number of Huh7 and HepG2 cells gradually slowed down,and the cell area and cell roundness decreased gradually.Conclusion PPⅠcan inhibit the proliferation and migration of Huh7 and HepG2 cells.
作者
喻青青
李征远
杜伯雨
YU Qingqing;LI Zhengyuan;DU Boyu(Institute of Biological Medicine,Hubei Medical University,Shiyan,Hubei 442000,China)
出处
《现代医药卫生》
2020年第22期3551-3554,共4页
Journal of Modern Medicine & Health
基金
湖北医药学院2018年研究生启动金资助计划项目(2018QDJR31)
湖北省十堰市科技局引导性项目(19Y16)。
