摘要
为了得到高产并符合生物柴油标准的微藻油脂的藻株,本文研究了在外源甘油存在的情况下野生型(WT)三角褐指藻(Phaeodactylum tricornutum),甘油激酶过表达藻株(GKOE)以及甘油激酶RNA干扰藻株(GKRNi)在添加浓度为20 mmol/L外源甘油的培养基中的生长情况、油脂产量以及脂肪酸组成.结果显示:(1)甘油兼养的GKOE藻细胞浓度可达到2.23×107个/mL,明显高于甘油兼养的WT的1.79×107个/mL和GKRNi的1.78×107个/mL;(2)甘油兼养的GKOE油脂含量可达到31.73%,比甘油兼养的WT和GKRNi分别高了27.20%和26.63%;(3)尼罗红染色显示甘油兼养的GKOE荧光强度比甘油兼养的WT和GKRNi分别高16.26%和17.83%;(4)甘油兼养的GKOE总脂肪酸产量可达到0.052 mg/mL,比甘油兼养的WT和GKRNi高了36.84%和10.64%,说明其油脂适合于生物柴油开发.
In order to obtain high-yield algae strains that meet biodiesel standards,the growth,lipid yield and fatty acid composition of Phaeodactylum tricornutum wild-type(WT),Phaeodactyllum tricornutum with glycerol kinase overexpression(GKOE)and glycerol kinase RNA interfered(GKRNi),which were cultured in a medium supplemented with 20 mmol/L exogenous glycerol,were investigated.The results showed that:(1)the cell concentration of GKOE in the medium with exogenous glycerol could reach 2.23×107/mL,significantly higher than WT(1.79×107/mL)and GKRNi(1.78×107/mL)in the medium with exogenous glycerol;(2)the lipid content of GKOE in the medium with exogenous glycerol was up to 31.73%,which was much higher than WT(27.20%)and GKRNi(26.63%)in the medium with exogenous glycerol;(3)the fluorescence intensity of GKOE in the medium with exogenous glycerol was 16.26%and 17.83%higher than WT and GKRNi in the medium with exogenous glycerol by Nile red staining,respectively;(4)the total fatty acid yield of GKOE reached 0.052 mg/mL,which was 16.26%and 17.83%higher than WT and GKRNi in the medium with exogenous glycerol,and suitable for biodiesel development.
作者
侯兴国
方琰
白欢
胡莎莎
卿人韦
兰利琼
HOU Xing-Guo;FANG Yan;BAI Huan;HU Sha-Sha;QING Ren-Wei;LAN Li-Qiong(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;Electric Power Research Institute of Sichuan Electric Power Company, Chengdu 610072, China)
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第6期1209-1216,共8页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金(40976092)
四川省电科院项目(13H1138)。
关键词
过表达甘油激酶三角褐指藻藻株
油脂
脂肪酸
生物柴油
Phaeodactyllum tricornutum with glycerol kinase overexpression
Lipid
Fatty acid
Biodiesel
