摘要
丁香精油是一种天然的高效抑菌剂,但是由于其水溶性差,限制了其广泛应用。研究以软脂酸(PA)为疏水基团,以N-乙酰-L半胱氨酸(NAC)为亲水基团,合成了具有两亲性的壳聚糖衍生物(PA-CS-NAC),并进一步制备了丁香精油-两亲性壳聚糖载药纳米胶束(NMs)。傅里叶红外光谱和核磁共振氢谱表明,PA和NAC都成功接枝到壳聚糖上。扫描电镜结果显示,NMs的形貌呈现分散均匀的圆球状。研究结果表明,丁香精油-两亲性壳聚糖载药纳米胶束在室温条件下具有pH响应性能,其对灰葡萄孢菌的抑菌性能较未包埋的丁香精油提高10%以上,抑菌性能显著提高。
Clove essential oil is a natural and highly effective bacteriostatic agent,but its poor water solubility limits its wide application.In this study,palmitic acid(PA)was used as the hydrophobic group and N-acetyl-L cysteine(NAC)was used as the hydrophilic group to synthesize amphiphilic chitosan derivatives(PA-CS-NAC),and further prepared clove essential oil-amphiphilic chitosan drug-loaded nanomicelles(NMs).Fourier infrared spectroscopy and nuclear magnetic resonance hydrogen spectroscopy showed that both PA and NAC were successfully grafted onto chitosan.Scanning electron microscopy results showed that the morphology of NMs showed a uniformly dispersed spherical shape.The research results have shown that clove essential oil-amphiphilic chitosan-loaded nanomicelles had pH-responsive performance at room temperature,and their antibacterial properties against Botrytis Cinerea increased by more than 10%compared with unembedded clove essential oil,where the performance of bacteria was improved significantly.
作者
何祖宇
李普旺
杨子明
王超
刘运浩
姚金胜
宋书会
周闯
HE Zuyu;LI Puwang;YANG Ziming;WANG Chao;LIU Yunhao;YAO Quansheng;SONG Shuhui;ZHOU Chuang(South Subtropical Crop Research Institute of Chinese Academy of Tropical Agricultural Sciences,key Laboratory of Hainan Province for Postharrest Physiology and Technology ofTrpical Horticultural Products, Zhanjiang 524091, China;Agricultural Product Processing Research Institute of Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524001, China)
出处
《功能材料》
EI
CAS
CSCD
北大核心
2020年第11期11182-11188,共7页
Journal of Functional Materials
基金
海南省自然科学基金资助项目(219QN294,319QN297)
中国热带农业科学院基本科研业务费专项资助项目(1630122020004,1630122017009)
云南省李普旺专家工作站资助项目(2020005AF150007)
广东省基础与应用基础研究基金资助项目(2018A030313109,2019A1515010714)
广东省现代农业产业技术体系创新团队建设专项资金资助项目(2019KJ140)
湛江市科技项目资助项目(2018A02015)。
关键词
壳聚糖
两亲性
纳米胶束
灰葡萄孢菌
抗菌
chitosan
amphiphilic
nanomicelles
botrytis cinerea
antibacterial
