摘要
目的:观察三七总皂苷对肝癌细胞HepG2的钙离子平衡和生长的影响。方法:用50、100、200、400、800 mg/L的三七总皂苷药物溶液处理肝癌细胞HepG2(北纳生物公司),加上正常对照组,共6个分组。培养各组细胞,设置24、48、72 h 3个时间点,噻唑蓝(MTT)检测肝癌细胞抑制率,流式细胞仪检测细胞凋亡,用Fura-2 AM钙离子荧光探针染色后激光共聚焦显微镜测定细胞内钙离子浓度。采用One-way ANOVA检验。结果:随着药物浓度升高和药物作用时间延长,HepG2细胞相对生长抑制率[50 mg/L组:(11.01±2.54)%、(14.46±2.61)%、(19.03±4.20)%;100 mg/L组:(16.97±4.75)%、(21.61±2.85)%、(27.71±4.54)%;200 mg/L组:(22.96±4.08)%、(28.31±2.36)%、(35.61±5.04)%;400 mg/L组:(30.89±4.01)%、(41.61±6.18)%、(51.77±8.32)%;800 mg/L组:(37.96±3.90)%、(49.82.46±5.81)%、(62.23±5.70)%](F=44.986、67.821、56.230,P<0.05),差异有统计学意义;在24、48 h处理组中,随着药物浓度升高,细胞凋亡率也随之升高,与对照组比较差异有统计学意义(F=79.784、77.350,P<0.05),并以三七总皂苷浓度在200 mg/L及以上时凋亡率更为明显;在24、48、72 h处理组中可见,随着药物浓度增加,荧光强度升高逐渐明显,即细胞内Ca 2+浓度逐渐增加,各组间对比差异有统计学意义(F=26.087、75.007、199.334,P<0.05)。结论:三七总皂苷能够抑制人肝癌细胞HepG2的生长,诱导其凋亡,并且具有浓度和时间依赖,其机制可能是通过影响HepG2细胞内钙离子浓度来起到肿瘤抑制作用。
Objective To observe the influence of panax notoginseng saponin(PNS)on the growth and the Ca+balance of HepG2 cells.Methods The HepG2 cells were treated with 50,100,200,400,800 mg/L PNS,and the normal control group was created.Culturing the 6 groups of HepG2 cells,proceed the experiments in 3 timing of 24,48,72 h.Detect the cell proliferation by methyl thiazolyl tetrazolium(MTT)assay,detect the cell apoptosis by flow cytometry,and detect the Ca+content in cell with Fura-2 AM probe by CLSM.Results With the increase of drug concentration and the prolongation of drug action time,the relative growth inhibition rate of HepG2 cells[50 mg/L group:(11.01±2.54)%,(14.46±2.61)%,(19.03±4.20)%;100 mg/L group:(16.97±4.75)%,(21.61±2.85)%,(27.71±4.54)%;200 mg/L group:(22.96±4.08)%,(28.31±2.36)%,(35.61±5.04)%;400 mg/L group:(30.89±4.01)%,(41.61±6.18)%,(51.77±8.32)%;800 mg/L group:(37.96±3.90)%,(49.82.46±5.81)%,(62.23±5.70)%](F=44.986,67.821,56.230,P<0.05),the difference in the control group was statistically significant.In the 24 h and 48 h treatment group,the higher the concentration of total saponins of Panax notoginseng,the higher the apoptotic rate.The difference in the control group was statistically significant(F=79.784,77.350,P<0.05),and the apoptotic rate was more obvious when the total saponin concentration of Panax notoginseng was above 200 mg/L.In the 24,48,72 h treatment group,with the increase of drug concentration,the fluorescence intensity increased gradually,that is,the intracellular Ca2+concentration increased gradually,the difference between the groups was statistically significant(F=26.087,75.007,199.334,P<0.05).Conclusion Panax notoginseng saponins can inhibit the growth of human hepatoma cell HepG2 and induce apoptosis,and it is concentration-and time-dependent.The mechanism may be to inhibit tumors by affecting the intracellular calcium concentration of HepG2 cells.
作者
魏剑锋
许泽波
王伟
陈少坚
蔡少阳
许世堂
Wei Jianfeng;Xu Zebo;Wang Wei;Chen Shaojian;Cai Shaoyang;Xu Shitang(Department of Hepatobiliary Surgery,the Second Affiliated Hospital,Fujian Medical University,Quanzhou 362000,China;Department of Emergency,the Second Affiliated Hospital,Fujian Medical University,Quanzhou 362000,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2020年第10期1815-1818,共4页
Chinese Journal of Experimental Surgery
基金
泉州市科技局高层次人才创新创业项目(2017Z005)。
关键词
癌
肝细胞
三七总皂苷
HEPG2
钙离子平衡
Carcinoma,hepatocellular
Panax notoginseng saponin
HepG2
Ca2+balance
