摘要
益生菌在临床上防治动物腹泻有显著效果,为探讨其作用机制,将早期断奶仔猪分为2组,每组10头,对照组饲喂基础日粮,试验组在基础日粮中添加1‰复合益生菌,试验期60 d,期间观察各实验动物腹泻情况并记录腹泻指数,试验结束时每组随机选4头取盲肠食糜及结肠组织,利用Illumina MiSeq高通量测序分析盲肠内容物微生物结构,并用荧光定量PCR方法检测结肠组织TLR/MyD88/NF-κB信号通路相关基因转录水平。结果显示,试验组肠道菌群丰度与对照组比较有很大变化:在门水平上,试验组拟杆菌门(Bacteroidetes)平均丰度显著降低了7.05%(P<0.05),变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)平均丰度提高了4.28%,1.73%,1.50%;在属水平上,试验组乳杆菌属(Lactobacillus)的平均丰度显著降低了18.85%(P<0.05),而瘤胃球菌属(Ruminococcaceae)的平均丰度显著提高了8.79%(P<0.05),肠杆菌属(Enterobacteriaceae)、梭菌属(Clostridiales)、毛螺菌科(Lachnospiraceae)、链球菌属(Streptococcus)的平均丰度也分别上升了4.02%,3.94%,3.39%,3.01%。同时,试验组IL-10的表达量显著高于对照组(P<0.05),而MyD88、TLR4、NF-κB、IL-6、IL-8、TNF-α表达量均显著低于对照组(P<0.05)。试验组腹泻指数较对照组均有所下降,其中试验15,45 d差异显著(P<0.05)。可见复合益生菌增加了肠道微生物的丰度及有益菌的含量,并通过下调TLR/MyD88/NF-κB信号通路的转录抑制肠道炎症的发生,可有效防治动物腹泻。
probiotics have a significant effect on the prevention and treatment of animal diarrhea.In order to explore its mechanism,early weaned piglets were divided into two groups:control group(n=10) and experimental group(n=10).The experimental group was supplemented with 1 ‰ compound probiotics in the basic diet.During the experiment period of 60 days,the diarrhea index of each experimental animal was recorded.At the end of the experiment,4 cecal chyme and colon tissues were randomly selected from each group.Illumina MiSeq high throughput sequencing was used to analyze the microbial structure of cecal content,and fluorescence quantitative PCR was used to detect the transcription level of genes related to TLR/MyD88/NF-κB signaling pathway in colon tissue.The results showed that the abundance of intestinal flora in the experimental group changed greatly compared with the control group.At the gate level,the average abundance of Bacteroidetes in the experimental group decreased by 7.05%(P<0.05).The average abundance of Proteobacteria,Actinobacteria and Firmicutes increased by 4.28%,1.73% and 1.50%,respectively.At the genus level,the average abundance of Lactobacillus was significantly decreased by18.85%,while that of Ruminococcaceae was significantly increased by 8.79%(P<0.05).The average abundance of Enterobacteriaceae,Clostridiales,Lachnospiraceae and Streptococcus also increased by 4.02%,3.94%,3.39%and 3.01%,respectively.At the same time,the expression of IL-10 in the experimental group was significantly higher than that of the control group(P <0.05),while the expression of MyD88,TLR4,NF-κB,IL-6,IL-8 and TNF-αin the experimental group was significantly lower than that of the control group(P<0.05).The diarrhea index of the experimental group was lower than that of the control group,and the difference was significant on the15 th and 45 th day(P<0.05).It can be seen that compound probiotics can increase the abundance of intestinal microorganisms and the content of beneficial bacteria,and inhibit the occurrence of intestinal inflammation by down-regulating the transcription of TLR/MyD88/NF-κB signaling pathway,which can effectively prevent and treat diarrhea in animals.
作者
刘士奇
陈建
查剑平
吴云辉
王仁文
郑丽红
王小莺
罗军荣
LIU Shi-qi;CHEN Jian;ZHA Jian-ping;WU Yun-hui;WANG Ren-wen;ZHENG Li-hong;WANG Xiao-ying;LUO Jun-rong(Jiangxi Province Key Laboratory of Diagnosis and Control of Livestock Diseases,Nanchang 330045,China;Jiangxi Zhongke Agriculture and Ani-mal Health Pharmaceutical Co.,Ltd.,Nanchang 330219,China;Yanshan County Agricultural and Rural Bureau,Yanshan,Jiangxi 334500,China;Institute of Animal Diseases Monitoring and Prevention,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2020年第10期2032-2039,共8页
Chinese Journal of Veterinary Science
基金
江西省教育厅资助项目(GJJ11397)
江西省科技支撑计划重点资助项目(20151BBF60046)。
关键词
复合益生菌
断奶仔猪
肠道菌群
高通量测序
炎症因子
compound probiotics
weanling piglets
intestinal flora
high throughput sequencing
inflammatory factors
