摘要
目的:对白头翁商品药材进行真伪鉴别研究,建立有效的检测方法。方法:分别采用生药学鉴别方法、HPLC特征图谱比较和主成分分析,对白头翁及其混伪品进行比较研究。结果:白头翁和非同属混伪品在性状、显微特征上有明显区别,但是和同属混伪品非常相似;通过建立HPLC特征图谱,可以区分出白头翁及其同属混伪品;结合主成分分析,能够更直观地显示出差别。结论:建立的系列检测方法可以用于白头翁及其混伪品的鉴定,方法简单易行,科学有效,并为近缘种易混中药材的鉴定提供参考。
Objective:To establish a valid method to identify Pulsatillae Radix and its adulterants.Methods:Pharmacognosy,HPLC characteristic chromatogram,principal component analysis(PCA) were used to distinguish Pulsatillae Radix and its adulterants.Results:There was a significant difference between Pulsatillae Radix and its noncongeneric adulterants in the morphologic characteristics and microscopic characteristic,while Pulsatillae Radix was similar to its congeneric adulterants in the morphologic characteristics and microscopic characteristic.Pulsatillae Radix and its congeneric adulterants could be distinguished by HPLC characteristic chromatogram combined with principal component analysis.The difference could be observed visually by PCA.Conclusion:Serial detection method is scientific and effective,which can be used to distinguish Pulsatillae Radix and its adulterants.It also gives an example for identification of sibling species traditional Chinese medicine.
作者
张璐
田静
尹萌
程显隆
魏锋
马双成
ZHANG Lu;TIAN Jing;YIN Meng;CHENG Xian-long;WEI Feng;MA Shuang-cheng(Yangzhou Center for Food and Drug Control,Yangzhou 225000,China;National Institutes for Food and Drug Control,Beijing 100050,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2020年第10期1808-1819,共12页
Chinese Journal of Pharmaceutical Analysis
基金
江苏省市场监督管理局科技计划项目"白头翁质量标志物及质量评价研究"(KJ207563)。
关键词
白头翁
真伪鉴别
生药学
特征图谱
主成分分析
Pulsatillae Radix
authentic identification
pharmacognosy
characteristic chromatogram
PCA
