摘要
[目的]了解黄芩(Scutellaria baicalensis Georgi)种子萌发过程中的初生与次生代谢规律。[方法]超氧化物岐化酶(SOD)活性测定采用核黄素-NBT法,过氧化物酶(POD)活性测定采用愈创木酚比色法,过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、苯丙氨酸解氨酶和肉桂酸-4-羟化酶活性测定采用紫外分光光度法。次生代谢物质含量测定采用高效液相色谱法。[结果]在黄芩种子萌发的前5 d,可溶性糖含量不断降低,当子叶形成时有所增高。苯丙氨酸解氨酶、肉桂酸-4-羟化酶和查耳酮合成酶均随着萌发的进行而增加,其次生代谢物质也呈现出相同的变化趋势。并且次生代谢物质与关键酶的活性呈现正相关。[结论]在种子萌发的过程中次生代谢物质的形成与关键酶呈现显著相关,所以要想获得高质量的药材可以通过采取措施提高关键酶的活性。
[Objective]The aim of this study was to study the plant metabolism during the seed germination of Scutellaria baicalensis Georgi.[Method]The activities of SOD and POD were determined with lactoflavine-NBT method and guaiacol-colorimetry assay,respectively.The CAT,APX,PAL and C4H were determined with ultraviolet spectrophotometry.The secondary metabolites were detected by high performance liquid chromatography(HPLC).[Result]The results indicated that the soluble sugar content decreased during the first 5 days and then increased when the cotyledons formed.The PAL,C4H and CHS continuously increased with different stages of the seed germination.The secondary metabolites also increased during the seed germination.And the secondary metabolites had significant negative correlation with the key enzyme.[Conclusion]The secondary metabolites have significant negative correlation with the key enzyme.So we can improve the secondary metabolites by improving the key enzyme activity.
作者
刘金花
杨桂玲
刘谦
张永清
LIU Jin-hua(Jining Center for Food and Drug Control,Jining,Shandong 272000)
出处
《园艺与种苗》
CAS
2020年第11期1-4,29,共5页
Horticulture & Seed
基金
2019—2020年度山东省中医药科技发展计划项目(2019-1036)
2019年中医药公共卫生服务补助专项“全国中药资源普查项目”(财社[2019]39号)。
