摘要
目的基于高精度串联质谱技术的蛋白质组方法,对河流漫游球菌的蛋白质组组分进行全面鉴定,并对其生物学功能开展探讨。方法通过分离临床骨科患者深部组织穿刺液获得并鉴定得到一株河流漫游球菌,采用液相色谱-串联质谱系统开展无标定量蛋白质组实验,对河流漫游球菌进行蛋白质组组分鉴定,将分析所得的数据通过MaxQuant软件进行数据库检索完成蛋白质的鉴定和相对定量,并对鉴定出的蛋白质从生物学途径、分子功能和细胞组分,以及KEGG通路多个方面进行分类和注释分析。结果通过3次质谱重复实验,共鉴定得到河流漫游球菌的1372个蛋白质;通过功能注释分析发现大部分蛋白质主要参与维持细菌生存的转录翻译、代谢以及物质的生物合成等生物过程。结论河流漫游球菌蛋白质丰度排名前10的主要为酶和核糖体蛋白,其中高富集蛋白质烯醇化酶、磷酸甘油酸激酶为参与糖酵解功能的关键酶,并有可能与河流漫游球菌毒性密切相关。本研究构建的河流漫游球菌蛋白质组表达谱数据集为临床河流漫游球菌的诊断与治疗提供了新的蛋白质组实验数据参考。
Based on the high-precision mass spectrometry platform,the proteomics analysis was first applied on Vagococcus fluvialis to identify the protein components and explore the primary biological functions.The Vagococcus fluvialis was isolated from the infected puncture fluid of lower left femur from a patient in China.By using liquid chromatography-tandem mass spectrometry and label free quantification technology,the most comprehensive proteome profile of Vagococcus fluvialis was constructed,containing 1372 proteins.All mass spectrometry profiles were analyzed using the MaxQuant software;the identified proteins were then annotated according to biological processes,molecular functions and cellular components Gene Ontology,as well as KEGG pathway analysis.As results,most proteins are involved in the basic biological processes,including transcription,translation,metabolism and biosynthesis.The top ten proteins with most identified intensities are enzymes and ribosomal proteins,among which,the Enolase and Phosphoglycerate kinase are key enzymes involved in glycolysis,and could be involved in bacterial virulence.In summary,the proteome profile dataset constructed in this study would provide better understanding on pathogenic mechanisms of Vagococcus fluvialis at the level of protein expression.
作者
曹喻
周婷
段晓雷
陈泽慧
杨欢
闵迅
CAO Yu;ZHOU Ting;DUAN Xiao-lei;CHEN Ze-hui;YANG Huan;MIN Xun(Department of Laboratory Medicine of the Affiliated hospital,Zunyi Medical College,Zunyi 563003,China;School of Laboratory Medicine,Zunyi Medical College,Zunyi 563000,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2020年第11期900-906,共7页
Chinese Journal of Zoonoses
基金
贵州省联合基金资助项目(No.黔科合LH字[2017]7097)。
关键词
河流漫游球菌
质谱
蛋白质组
功能注释
Vagococcus fluvialis
mass spectrometry
proteomics
function annotation
