摘要
为研究波罗蜜叶绿素缺失突变体(CDM)次生生长茎中抗病基因(PRGs)的变化,从转录组数据中筛选出9 630个PRGs。去除低丰度(FPKM≤0.5)后进行差异表达基因(DEGs)筛选,将2 513个DEGs进行加权基因共表达网络分析(WGCNA),寻找到2个与表型关联性较高的模块,共183个DEGs。对模块基因进行GO功能注释、分类、转录因子(TFs)及类受体蛋白激酶(RLKs)分析。结果显示,模块基因主要注释在结合(Binding)、细胞过程(Cellular process)和催化活性(Catalytic activity)等功能组。TFs分析显示,共有7个家族,14个转录因子。RLKs分析发现,RLK-Pelle-DLSV亚家族的12个DEGs中有11个在CDM中上调表达,说明该亚家族在波罗蜜CDM次生生长茎抵御逆境侵害过程中发挥着重要作用。本研究运用转录组测序数据分析波罗蜜CDM次生生长茎中PRGs的变化,为光合作用对木本植物茎响应逆境胁迫的研究提供数据。
In order to study the changes of plant resistance genes(PRGs) in the secondary growth stems of chlorophyll deficient mutant(CDM) of A. heterophyllus, 9 630 PRGs were selected from the transcriptome data. After removing the low abundance(FPKM≤0.5), the differentially expressed genes(DEGs) were screened, and 2 513 DEGs were subjected to weighted gene coexpression network analysis(WGCNA). There were 183 DEGs in the modules with high type correlation. The module genes were analyzed for GO function annotation, classification,transcription factors(TFs) and receptor-like kinases(RLKs). The results showed that module genes were mainly annotated in functional groups such as binding, cellular process, and catalytic activity. TFs analysis showed that there were 7 families, 14 transcription factors. RLKs analysis found that 11 of the 12 DEGs in the RLK-Pelle_DLSV subfamily were up-regulated in CDM, indicating that the subfamily played an important role in resisting stress of secondary growth stems of A. heterophyllus CDM. This study used transcriptome sequencing data to analyze the changes of PRGs in the secondary growth stems of A. heterophyllus CDM, providing data for the study of photosynthesis in response to stress of woody plant stems.
作者
郑李婷
于旭东
蔡泽坪
罗佳佳
吴繁花
董俊娜
谢柳青
Zheng Liting;Yu Xudong;Cai Zeping;Luo Jiajia;Wu Fanhua;Dong Junna;Xie Liuqing(Key Laboratory of Geneties and Germplasm Inovation of Tropical Special Forest Trees and Omamental Pants,Ministy of Education,College of Forestry,Hainan University,Haikou,570228;College of Tropical Crops,Hainan University,Haikou,570228;Key Labontory of Crop Gene Re-sources and Germplasm Enhancement in Southerm China,Ministry of Agricuture,Topical Crops Genetie Resources Isitute,Chinese Acndemy of Tropical Agriculural Sciemces,Danzhou 571737;School of Life and Phanmaecutical Scicnce,Hainan University,Hakou,570228)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第22期7348-7355,共8页
Molecular Plant Breeding
基金
海南省自然科学基金项目(319MS017)
现代农业产业技术体系建设专项资金(CARS-11-HNCYH)
海南大学科研启动基金项目(kyqd1620)共同资助。
