摘要
目的探讨肥胖糖尿病酮症酸中毒(DKA)患者血清白细胞介素(IL)-6、C反应蛋白(CRP)和同型半胱氨酸(Hcy)变化及意义。方法收集2018年9月至2019年5月在内分泌科住院且诊断为DKA的患者50例为研究对象,同时选取健康受试者25例为对照组,根据体质指数(BMI)将DKA患者分为肥胖组25例(BMI≥25 kg/m^2)和非肥胖组25例(BMI<25 kg/m^2)。采用OPTI血气分析仪分析动脉血气[pH、碳酸氢根(HCO3-)],采用Luminex 200多功能流式点阵仪检测IL-6水平,AU2700全自动生化分析仪检测Hcy和血糖,采用BNProSpec特定蛋白分析仪检测CRP水平。结果与入院时相比,肥胖组和非肥胖组DKA患者经胰岛素治疗24 h后血糖水平显著降低,pH及HCO3-均显著升高(P<0.01)。与对照组相比,肥胖组及非肥胖组DKA患者入院时血清IL-6、CRP及Hcy水平显著升高(P<0.05);与入院时相比,肥胖组及非肥胖组DKA患者在胰岛素治疗24 h后IL-6、CRP及Hcy水平显著降低(P<0.01),但肥胖组DKA患者IL-6、CRP及Hcy仍高于对照组(P<0.05)。结论肥胖组DKA患者血清IL-6、CRP和Hcy升高,在疾病过程中发挥重要作用。
Objective T o explore the changes and significance of serum interleukin(IL)-6,C-reactive protein(CRP)and homocysteine(Hcy)in obese patients with diabetic ketoacidosis(DKA).Methods Fifty patients who were hospitalized in the department of endocrinology and were diagnosed with DKA from September 2018 to May 2019 were collected as the research objects.At the same time,25 healthy subjects were selected as the control group.According to the body mass index(BMI),the DKA patients were divided into obese DKA group(BMI≥25 kg/m^2,n=25)and non-obese DKA group(BMI<25 kg/m^2,n=25).OPTI blood gas analyzer was used to analyze arterial blood gas(pH,HCO3-),Luminex200 was used to detect IL-6 levels,AU2700 was used to detect Hcy and serum glucose levels,and BNProSpec specific protein analyzer was used to detect CRP levels.Results Compared with the time of admission,the serum glucose of obese and non-obese DKA patients were significantly reduced after insulin treatment for 24 hours,and the pH and HCO3-were significantly increased(P<0.01).Compared with the control group,the serum IL-6,CRP and Hcy levels of obese and non-obese DKA patients were significantly increased upon admission(P<0.05).Compared with the time of admission,the levels of IL-6,CRP and Hcy in obese and non-obese DKA patients were significantly lower after 24 hours of insulin treatment(P<0.01),but the levels of IL-6,CRP and Hcy in obese DKA patients were still high than those of the control group(P<0.05).Conclusion The levels of serum IL-6,CRP and Hcy in obese DKA patients play an important role in the disease process.
作者
严开斌
石巍
YAN Kai-bin;SHI Wei(Department of Laboratory,Hongze Huaian District People's Hospital,Huai'an,Jiangsu 223100,China)
出处
《中国临床研究》
CAS
2020年第11期1533-1536,共4页
Chinese Journal of Clinical Research
