摘要
【目的】采用微生物法解决土壤中残留的菊酯类农药问题。【方法】通过唯一碳源法从土壤样本中筛选出发菌株,采用紫外分光光度法及HPLC法对菌株降解能力进行测定,用形态学及ITS测序对菌株种属进行鉴定,通过菌丝生长及产孢量的测定观察该菌株的生长特性,采用摇瓶及室内土壤降解试验验证其降解能力。【结果】从土壤中获得一株高效分解利用菊酯类农药的微生物菌株SSCL-3,经鉴定该菌为米曲霉(Aspergillus oryzae),该菌株可在含1000 mg/L的高效氯氰菊酯无机盐培养基中正常生长。对多种高浓度菊酯类农药耐受。米曲霉SSCL-3在无机盐培养基、28℃、150 r/min摇瓶培养24 h的条件下,对500 mg/L高效氯氰菊酯的降解率为88.9%。土壤室内试验证明该菌在土壤中,温度20~34℃、水分含量保藏40%~60%条件下,30 d可将土壤中400 mg/L的高效氯氰菊酯降解45.6%。【结论】该菌株或可解决土壤残留菊酯类农药问题。
【Objective】The present paper aimed to find solution of pyrethroid residues in soil with microbial method.【Method】Single carbon source culture was used to screen out strains.The degradation ability of the strain was determined by UV spectrophotometry and HPLC.The strain was identified by morphology and its ITS sequencing analysis.Growth characteristics were observed by mycelial growth and sporulation.The degradation ability was verified by shaking flask and soil test in lab.【Result】A strain named SSL-3 was isolated from soil samples,which could utilizeβ-Cypermethrin as carbon and energy source efficiently.This strain normally growth in mineral medium whichβ-Cypermethrin concentration at 1000 mg/L.Strain SSCL-3 was identified as Aspergillus oryzae.The strain SSCL-3 was multi-resistant to pyrethroid in highly concentration.88.9%ofβ-Cypermethrin(500 mg/L)in mineral medium could be degraded at 28℃within 24 hours.Laboratory soil tests showed thatβ-cypermethrin in soil(400 mg/L)could be degraded 45.6%in 30 days which kept temperature form 20℃to 34℃and moisture content from 40%to 60%.【Conclusion】The strain SSCL-3 may solve the problem of pyrethroid residues in soil.
作者
陈锐
门欣
瞿佳
邓媛
赵玲侠
孙晓宇
沈卫荣
CHEN Rui;MEN Xin;QU Jia;DENG Yuan;ZHAO Ling-xia;SUN Xiao-yu;SHEN Wei-rong(Shaanxi Institute of Microbiology,Microbial Resources Research Center,Shaanxi Xi'an 710043,China;Engineering Center of Qinling Mountains Natural Products,Shaanxi Academy of Sciences,Shaanxi Xi’an 710043,China)
出处
《西南农业学报》
CSCD
北大核心
2020年第10期2274-2280,共7页
Southwest China Journal of Agricultural Sciences
基金
陕西省科学院科技计划项目(2018K-08)
陕西省科学院科技计划项目(2018NK-01)。
关键词
真菌
米曲霉
高效氯氰菊酯
生物降解
Fungi
Aspergillus oryzae
β-Cypermethrin
Biodegradation
