摘要
气候变化引发干旱频度和强度的变化影响植物的生长发育和生态适应。干旱胁迫会引起木质部栓塞并造成水力失效,而如何准确量化木质部抗栓塞的能力对研究植物对干旱的响应过程尤为重要。通常可通过脆弱性曲线量化木质部抗栓塞的能力。目前已经研发出构建木质部栓塞脆弱性曲线的多种方法,但不同方法往往产生不一致的结果。深入理解这些方法的设计原理并在实际应用时比较各方法的异同,对合理解释相关文献数据及准确选择干旱预测模型等尤为重要。本文阐述了自然干燥法、离心法、注气法、声学测定法、同步加速器与X射线显微断层扫描法、光学可视化法及抽气法7种测定木质部栓塞脆弱性的方法,并总结了近年来各测定方法在具体研究中的运用情况及存在的争议。最后,对未来研究测定木质部栓塞脆弱性与实际运用相关方法的选择等提出了展望。
Changes in the frequency and severity of drought events associated with climate change could affect plant growth, development, and adaptability. Hydraulic failure caused by xylem embo-lism is the main physiological consequences of drought stress. How to accurately quantify xylem embolism is particularly important for understanding plant responses to drought stress. The vulnerability of xylem to embolism is usually evaluated by constructing vulnerability curves(VCs). Several methods have been developed to construct VCs, but be inconsistent in their results. A deep understanding of the design principles of xylem embolism measurement methods and comparison of the similarities and differences of various methods in actual research are particularly important for the rational interpretation of literature results, and properly using VCs in models for predicting plant responses to water deficits. Here, we compared seven methods for constructing xylem vulnerability curves to embolism: bench dehydration, centrifugation, air injection, acoustic measurements, synchrotron and X-ray microtomography(Micro-CT), optical visualization method, and pneumatron method. We summarized current achievements and controversial viewpoints of the application of these methods in specific research. Finally, we provided prospects for measuring the vulnerability of xylem embolism and the selection of relevant methods for practical application in future studies.
作者
王婷
郭雯
潘志立
陈芳
杨石建
WANG Ting;GUO Wen;PAN Zhi-li;CHEN Fang;YANG Shi-jian(School of Ecology and Environmental Science,Yunnan University,Kunming 650500,China;Yunnan Province Key Labora-tory of Plant Reproductive Adaption and Evolutionary Ecology,Kunming 650500,China;Institute of Economic Plant,Yunnan University,Kunming 650504,China)
出处
《应用生态学报》
CAS
CSCD
北大核心
2020年第11期3895-3905,共11页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(31760114,31800334)
云南省科技厅基础研究计划项目(2019FD004)
云南大学“东陆中青年骨干教师”培养计划项目(2018YDDLQN69)、云南大学“双一流”建设项目省优秀青年培育项目(2018YDJQ024)和云南大学研究生科研创新基金项目(2019z051)资助。
关键词
气穴化
木质部栓塞
脆弱性曲线
耐旱
cavitation
xylem embolism
vulnerability curve
drought tolerance
