摘要
目的:探讨恶性胸腔积液中巨噬细胞血红蛋白清道夫受体(CD163)、血管生成素样蛋白4(ANGPTL4)表达情况及两者的相关性。方法:收集2014年1月~2015年11月在我科住院治疗的40例初诊肺癌伴恶性胸腔积液(MPE)及20例结核性胸腔积液(TPE)患者胸水,免疫组化法检测胸水巨噬细胞CD163及ANGPTL4表达,计算阳性细胞百分比;ELISA法检测胸水上清液ANGPTL4浓度。结果:MPE组CD163+巨噬细胞中位数(97.32%)多于TPE组(26.41%)(P<0.05);MPE组所有巨噬细胞均表达ANPTL4,TPE组表达ANGPTL4的巨噬细胞占(76.5±14.37)%;MPE组ANGPTL4浓度中位数(40.50 ng/mL)高于TPE组(27.25 ng/mL)(P<0.05);MPE组CD163+巨噬细胞百分比与ANGPTL4浓度呈高度正相关(Rs=0.912,P<0.01)。结论:CD163+巨噬细胞可能参与肺癌MPE形成过程,ANGPTL4可能是其中重要介质。
Objective:To investigate the expression and correlation of macrophage hemoglobin scavenger receptor(CD163)and angiogenin-like protein 4(ANGPTL4)in malignant pleural effusion.Methods:Pleural fluid was collected from 40 newly diagnosed lung cancer patients with malignant pleural effusion(MPE)and tuberculous pleural effusion(TPE)in 20 patients treated in our department between January 2014 and November 2015.Immunohistochemistry was used to determine the expression of CD163 and ANGPTL4 in pleural macrophages,and the percentage of CD163 positive cells was calculated.ELISA was performed to measure ANGPTL4 concentration in pleural fluid.Results:The median concentration of CD163+macrophages was significantly higher in the MPE group(97.32%)than in the TPE group(26.41%)(P<0.05).ANPTL4 was totally expressed in all macrophages in the MPE group,and in(76.5±14.37)%of macrophages in the TPE group.The median concentration of ANGPTL4 was significantly higher in the MPE group(40.50 ng/mL)than in the TPE group(27.25 ng/mL)(P<0.05).The percentage of CD163+macrophages in MPE group were highly positively correlated with the concentration of ANGPTL4(Rs=0.912,P<0.01).Conclusion:CD163+macrophages may be involved in the formation of MPE in lung cancer,and ANGPTL4 may be an important mediator.
作者
秦立龙
臧蕾蕾
全斌
牛永亮
刘伟
潘玲玲
孙珍贵
QIN Lilong;ZANG Leilei;QUAN Bin;NIU Yongliang;LIU Wei;PAN Lingling;SUN Zhengui(Department of Respiratory Disease,The First Affiliated Hospital of Wannan Medical College,Wuhu 241001,China)
出处
《皖南医学院学报》
CAS
2020年第6期528-531,共4页
Journal of Wannan Medical College
基金
安徽省自然科学基金项目(1608085MH192)。
