摘要
目的探讨蜂毒肽Melittin突变体Melittin-K1逆转人肝癌耐药细胞株BEL-7402/5-FU多药耐药性的作用及机制。方法采用CCK-8法检测细胞活率评价Melittin-K1对BEL-7402/5-FU细胞生长的影响以及Melittin-K1能否逆转BEL-7402/5-FU细胞的耐药性。实时荧光定量PCR技术对Melittin-K1处理后BEL-7402/5-FU细胞多药耐药基因MDR1表达的改变进行测定。流式细胞术检测细胞表面P-糖蛋白(P-glycoprotein,P-gp)的表达以及细胞内罗丹明-123的蓄积水平评价Melittin-K1对P-gp蛋白表达及其功能的影响。结果细胞体外增殖实验结果表明,Melittin-K1显著抑制BEL-7402/5-FU细胞生长。Melittin-K1下调BEL-7402/5-FU细胞MDR1基因的表达并抑制细胞膜表面P-gp的表达水平及功能。结论新型多肽Melittin-K1具有逆转肝癌耐药细胞株BEL-7402/5-FU多药耐药性的作用。
Objective To investigate the mechanism by which Melittin-K1 reverses multidrug resistance of BEL-7402/5-FU cells.Methods CCK-8 assay was used to evaluate the effect of Melittin-K1 on the growth of BEL-7402/5-FU cells and to explore whether Melittin-K1 could reverse the drug resistance of BEL-7402/5-FU cells.The expression of MDR1 mRNA level was detected by real-time fluorescence quantitative PCR assay.The flow cytometry was used to measure the expression of P-glycoprotein(P-gp)on the cell membrane surface and the accumulation of rhodamine-123 in cells.Results Melittin-K1 significantly inhibited the growth of BEL-7402/5-FU cells in vitro in a time and dose-dependent manner.Melittin-K1 suppressed the level of MDR1 mRNA and inhibited the surface expression and function of P-gp in BEL-7402/5-FU cells.Conclusions Melittin-K1,a novel peptide,exhibited the activity of reversing multidrug resistance of liver cancer cells.
作者
董健
赵诗迪
陈婷瑶
柯梦云
吕毅
Dong Jian;Zhao Shidi;Chen Tingyao;Ke Mengyun;Lyu Yi(National Local Joint Engineering Research Center for Precision Surgery&Regenerative Medicine,Shanxi Provincial Center for Regenerative Medicine and Surgical Engineering,First Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,China;Department of Vascular Surgery,First Affiliated Hospital of Xi′an Jiaotong University,Xi′an 710061,China;School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China)
出处
《中华普通外科杂志》
CSCD
北大核心
2020年第11期882-886,共5页
Chinese Journal of General Surgery
关键词
抗菌肽类
化学疗法
辅助
多药耐药
P-糖蛋白
Dermcidins
Chemotherapy,adjuvant
Multidrug resistance
P-glycoprotein
