摘要
利用土壤宏基因组Illumina MiSeq第二代高通量测序,对大连星海湾路段绿化带土壤壳聚糖调节后根际菌群进行了测定分析,以期探究壳聚糖土壤调节剂对绿化带植物根际土壤微生物群落结构的影响,进而对改善绿化带土壤肥力、调节根际菌群、促进绿化带植物生长产生积极作用。结果表明:与对照组相比,壳聚糖处理后金叶榆新梢长生长速度显著提高。土壤理化性质并没有得到明显改善,然而微生物群落发生了较大的变化。壳聚糖调节后,土壤微生物物种丰富度和多样性指数提高。细菌主要聚类在变形菌门、酸杆菌门、拟杆菌门、放线菌门、疣微菌门、浮霉菌门和奇古菌门这7个菌门;真菌主要聚集在子囊菌门、担子菌门、被孢霉门、球囊菌门和隐真菌门这5个菌门。在属级别上共检测到473个细菌菌属和379个真菌菌属,其中Gp4、亚硝化螺菌属(Nitrososphaera)、芽单胞菌属(Gemmatimonas)、镰刀菌(Fusarium)、未知-座囊菌(unclassified_Dothideomycetes)、被孢霉菌(Mortierella)等优势菌属在壳聚糖处理后丰度上调;Terrimonas、Subdivision3_genera_incertae_sedis、Luteimonas、Endophoma、粉褶蕈属(Entoloma)、曲霉属(Aspergillus)等真菌在壳聚糖处理后丰度下调。壳聚糖处理后,氧化磷酸化、硫胺素新陈代谢和脂质代谢功能上调。壳聚糖作为一种天然土壤调节剂促进了绿化带植物金叶榆的新梢生长,改善了土壤微生物多样性、群落结构和组成。
Soil macrogenome Illumina MiSeq,the second generation of high-throughput sequencing,was used to determine and analyze the rhizosphere microflora adjusted by soil chitosan in the greenbelt of Xinghaiwan road,Dalian.In order to explore the influence of chitosan soil regulator on the soil microbial community structure in the rhizosphere of plants in the green belt,and then to improve soil fertility,regulate rhizosphere bacteria and promote the growth of plants in the green belt.The results showed that,compared with the control group,the growth rate of new shoots of elm was significantly increased after chitosan treatment.The physical and chemical properties of soil were not improved obviously,but the microbial community changes greatly.The soil microbial species richness and diversity index increased after chitosan adjustment.The bacteria were mainly clustered in the seven phylum of Proteobacteria,Acidobacteria,Bacteroidetes,Actinobacteria,Verrucomicrobia,Planctomycetes and Thaumarchaeota.The fungi were mainly concentrated in the five phyla of Ascomycota,Basidiomycota,Mortierellomycota,Glomeromycota and Rozellomycota.A total of 473 bacteria and 379 fungi were detected at the genus level,among which Gp4,Nitrososphaera,Gemmatimonas,Fusarium,unclassified_Dothideomycetes,Mortierella and other dominant bacteria were up-regulated after chitosan treatment.The abundance of Terrimonas,Subdivision3_genera_incertae_sedis,Luteimonas,Endophoma,Entoloma,Aspergillus and other fungi get decreased after chitosan treatment.Oxidative phosphorylation,thiamine metabolism and lipid metabolism are upregulated after chitosan treatment.Chitosan,as a natural soil regulator,can promote the growth of new shoots and improved the soil microbial diversity,community structure and composition.
作者
张萌
孙明
王岩
张春光
蒋山
尹恒
ZHANG Meng;SUN Ming;WANG Yan;ZHANG Chunguang;JIANG Shan;YIN Heng(College of Transportation Engineering,Dalian Maritime University,Dalian Liaoning 116026;Natural Products&Glycoconjugate Research Group,Dalian Institute of Chemical Physics,Chinese Academy of Science,Dalian Liaoning 116026;College of Food Science and Engineering,Northwest A&F University,Yangling,Shanxi 712100;Sehool ofBiological Engineering,Dalian Polytechnic University,Dalian,Liaoning 116034)
出处
《北方园艺》
CAS
北大核心
2020年第22期86-95,共10页
Northern Horticulture
基金
国家重点研发计划资助项目(2018YFD0201202)
国家自然科学基金资助项目(31672077)
大连市重点研发计划资助项目(2018YF23SN069)。
关键词
绿化带
土壤微生物
壳聚糖
高通量测序
green belt
soil microorganisms
chitosan
high throughput sequencing
