摘要
目的探讨miR-34a-Sirt1-NRF2信号通路对脑缺血损伤后内源性神经干细胞增殖的影响。方法制备改良局灶性大脑中动脉栓塞(MCAO)脑缺血损伤模型大鼠,分别设为脑缺血1 d组(n=10)、脑缺血3 d组(n=10)、脑缺血7 d组(n=10)、白藜芦醇组(n=10),另设不作缺血处理的大鼠为对照组(n=10),应用逆转录聚合酶链反应(RT-PCR)检测各组大鼠室管膜下区miR-34a、沉默信息调节因子1(Sirt1)、核因子E2相关因子2(NRF2)的表达水平。结果与对照组比较,白藜芦醇组、脑缺血1 d组、脑缺血3 d组、脑缺血7 d组miR-34a mRNA相对表达量升高,Sirt1、NRF2蛋白相对表达量降低,差异有统计学意义(P<0.05)。与白藜芦醇组比较,脑缺血1 d组、脑缺血3 d组、脑缺血7 d组miR-34a mRNA相对表达量升高,Sirt1、NRF2蛋白相对表达量降低,差异有统计学意义(P<0.05)。与脑缺血1 d组比较,脑缺血3 d组、脑缺血7 d组miR-34a mRNA相对表达量升高,Sirt1、NRF2蛋白相对表达量降低,差异有统计学意义(P<0.05)。与脑缺血3 d组比较,脑缺血7 d组miR-34a mRNA相对表达量升高,Sirt1、NRF2蛋白相对表达量降低,差异有统计学意义(P<0.05)。结论miR-34a有可能通过调节Sirt1/NRF2通路来调控脑缺血损伤后内源性神经干细胞增殖过程。
Objective To explore the effect of miR-34a-Sirt1-Nrf2 signal pathway on the proliferation of endogenous neural stem cells after cerebral ischemia injury.Methods Rats with modified focal middle cerebral artery embolism(MCAO)cerebral ischemia were prepared,and they were set as cerebral ischemia 1 d group(n=10),cerebral ischemia 3 d group(n=10),cerebral ischemia 7 d group(n=10),resveratrol group(n=10),and rats without ischemic treatment were also set up as control group(n=10).The expression levels of mir-34a,Sirt1,NRF2 in the subventricular zone of rats in each group were detected by RT-PCR.Results Compared with the control group,the relative expression of miR-34a mRNA in the resveratrol group,cerebral ischemia 1 d group,cerebral ischemia 3 d group and cerebral ischemia 7 d group increased,while the relative expression of Sirt1 and NRF2 protein decreased,the differences were statistically significant(P<0.05).Compared with the resveratrol group,the relative expression of miR-34a mRNA in the cerebral ischemia 1 d group,the cerebral ischemia 3 d group and the cerebral ischemia 7 d group increased,while the relative expression of Sirt1 and NRF2 protein decreased,the differences were statistically significant(P<0.05).Compared with the cerebral ischemia 1 d group,the relative expression of miR-34a mRNA in the cerebral ischemia 3 d group and the cerebral ischemia 7 d group increased,and the relative expression of Sirt1 and NRF2 protein decreased,the differences were statistically significant(P<0.05).Compared with the cerebral ischemia 3 d group,the relative expression of miR-34a mRNA in the cerebral ischemia 7 d group increased,while the relative expression of Sirt1 and NRF2 protein decreased,and the differences were statistically significant(P<0.05).Conclusion miR-34a may regulate the proliferation of endogenous neural stem cells after cerebral ischemic injury by regulating Sirt1/NRF2 pathway.
作者
廖伟
蔡崇明
王海彬
程伟
曾照彬
杨少春
LIAO Wei;CAI Chong-ming;WANG Hai-bin;CHENG Wei;ZENG Zhao-bin;YANG Shao-chun(Department of Neurosurgery,the First Aiffiliated Hospital of Gannan Medical University,Jiangxi Province,Ganzhou341000,China)
出处
《中国当代医药》
2020年第33期29-31,35,共4页
China Modern Medicine
基金
江西省卫生健康委科技计划项目(20204477)
赣南医学院科研课题(YB201918)。
