摘要
感染性疾病是全球儿童致残和死亡的主要原因之一,早期快速精准诊断感染致病原并进行针对性治疗对患儿预后至关重要。分子生物学技术,特别是核酸检测技术的更新和进步极大推动了病原生物学精准检测的发展。核酸分子杂交技术、核酸扩增技术、基因芯片、测序技术、基于基因编辑的病原检测技术等不仅拓展了病原核酸检测的通量,还显著提高了检测灵敏性,已逐渐成为儿童感染性疾病诊断的重要检测手段。现对不同核酸分子检测技术方法、检测时间、检测效能及潜在卫生经济学效益等多方面进行评估,以期明确不同儿童感染性疾病病原检测的适宜策略,为临床医师的遴选提供参考。
Infectious diseases is one of the leading causes of disability and death in children worldwide.Rapid,accurate and early detection of causative pathogens can contribute to timely treatment and diagnosis and may improve the prognosis of children with infectious disease.The continuous improvement of techniques in molecular biology,especially nucleic acid-based detection,have driven the development of accurate identification of the pathogens.Molecular diagnostic tests based on the polymerase chain reaction,alternative nucleic acid amplification,gene microarray,next-generation sequencing as well as clustered regularly interspaced short palindromic repeats-based technologies have gradually become important auxiliary tools for screening infectious pathogen due to their depth and accuracy.In this paper,several nucleic acid-based detection technologies are compared in order to constitute an appropriate diagnostic strategy for the detection of infectious pathogens.
作者
曹清
张巧珍
朱阅
沈楠
Cao Qing;Zhang Qiaozhen;Zhu Yue;Shen Nan(Department of Infection,Shanghai Children′s Medical Center,Shanghai Jiaotong University School of Medicine,Shanghai 200127,China)
出处
《中华实用儿科临床杂志》
CSCD
北大核心
2020年第22期1689-1693,共5页
Chinese Journal of Applied Clinical Pediatrics
基金
上海交通大学转化医学协同创新中心合作研究项目(TM201927)。
关键词
感染性疾病
分子检测
卫生经济学
儿童
Infectious diseases
Molecular diagnostic tests
Health economics
Child
