摘要
目的探讨腐霉利对青春期雄性小鼠阴茎、睾丸和精子的损伤。方法将4周龄雄性ICR小鼠随机分为实验组和对照组,每组8只,以灌胃方式分别给予各实验组小鼠50(低剂量)、100(中剂量)和200 mg/kg(高剂量)腐霉利,对照组仅给予等体积大豆油;于第11天,颈椎脱臼处死小鼠,称体重,测量肛门与生殖器间距离(anogenital distance,AGD)、睾丸容积和阴茎长度,称量睾丸、附睾和阴茎的重量并计算脏器系数,而后将一侧睾丸及1/2阴茎组织置于4%多聚甲醛中固定用于组织学分析,另外一侧睾丸及剩余1/2阴茎组织用于检测雄激素含量和Caspase-3、Caspase-9、Caspase-12和Bax基因表达,同时随机选取一侧附睾用于精子活力、密度以及形态检测。结果各实验组小鼠体重、阴茎长度、睾丸容积均无明显改变,但高剂量腐霉利染毒组小鼠睾丸、附睾重量以及睾丸、附睾脏器系数均显著降低(P<0.05)。各实验组小鼠睾丸曲细精管出现不同程度变性、生精细胞损伤、管腔精子数目减少,并且中、高剂量染毒组可见睾丸间质细胞增生;但各组阴茎组织未见显著病理改变。中、高剂量染毒组小鼠精子密度低于对照组(P<0.05),精子畸形率显著高于对照组(P<0.01),各剂量染毒组精子活力均低于对照组(P<0.05);中、高剂量染毒组睾丸组织中睾酮含量均高于对照组(P<0.001),阴茎组织中各剂量染毒组睾酮含量差异无统计学意义(P>0.05)。此外,高剂量染毒组睾丸组织中促凋亡基因Caspase-3、Caspase-9、Caspase-12和Bax的表达均显著升高(P<0.05)。结论腐霉利可致青春期雄性小鼠睾丸组织结构和功能损伤、精子质量降低、某些促凋亡基因表达增加。
OBJECTIVE To investigate the damage of procymidone to penile,testis and sperm in adolescent male mice.METHODS 4-week-old male ICR mice were allocated randomly to the treatment groups and the control group,with 8 mice in each group.Procymidone was administered to mice by gavage with dose 50 mg/kg(low dose),100 mg/kg and 200 mg/kg,while the control group was given only an equal volume of soybean oil.On the 11th days,the mice were sacrificed by spinal dislocation,their body masses were weighed,and the anogenital distance(AGD),testicular volume and penis length were measured.Furthermore the weight of the testes,epididymis and penis were weighed,the organ coefficients were calculated,and then a testis on one side and 1/2 penis tissue were fixed in 4%paraformaldehyde and used for histology analysis.The testis on the other side and the remaining 1/2 penis were used to detect androgen content and Caspase-3,Caspase-9,Caspase-12 and Bax gene expression.At the same time,one epididymis was randomly selected to detect sperm motility,density and morphology.RESULTS The weight,penis length and testicular volume of mice in each experimental group did not change significantly,while the weight of testes and epididymis and testicular and epididymal coefficients of mice in the high-dose group decreased significantly(P<0.05).The testis seminiferous tubules of every treatment group showed different degrees of degeneration,spermatogenic cell damage,and decreased number of luminal sperm.Meanwhile,medium and high dose treatment groups showed hyperplasia of testicular stromal cells.However,there was no significant pathologic change in penile tissue in every treatment group.The sperm density of mice in the middle and high dose groups was lower than that of the control group(P<0.05),and the sperm deformity rate in the two groups was significantly higher than that of the control group(P<0.01),while the sperm motility of every dose treatment group was lower than that of the control(P<0.05).The testosterone in the testis of the middle and high dose groups were high than the control(P<0.001),while there was no statistically significant difference of the testosterone among all the groups in the penis(P>0.05).In addition,the expression of pro-apoptotic genes Caspase-3,Caspase-9,Caspase-12 and Bax in testis tissues of the high-dose group were significantly increased(P<0.05).CONCLUSION Procymidone can cause damage to the structure and function of testes,reduce sperm quality,and increase the expression of certain pro-apoptotic genes in adolescent male mice.
作者
王振
李锐
阎政礼
付虎
朱勇飞
Wang Zhen;Li Rui;Yan Zhengli;Fu Hu;Zhu Yongfei(Department of Preventive Medicine,Medical School,Hunan Normal University,Changsha 410013,China)
出处
《卫生研究》
CAS
CSCD
北大核心
2020年第6期949-954,共6页
Journal of Hygiene Research
基金
湖南省自然科学基金(No.2019JJ40194)。
