摘要
本文探究了姜黄素光动力对牡蛎脂质氧化水解酶的影响,旨在从酶的角度阐明姜黄素光动力减缓牡蛎脂质氧化水解的机理。通过检测牡蛎内源酶活性,筛选新鲜牡蛎中产脂质氧化水解酶的菌株、利用16S rDNA进行菌种鉴定,结合Illumina MiSeq高通量测序技术对新鲜牡蛎中的产酶菌株进行分析。结果表明:姜黄素光动力能够降低内源性脂肪氧合酶(LOX)、脂肪酶(Lipase)、磷脂酶A1(PLA1)、磷脂酶A2(PLA2)、磷脂酶D(PLD)的活性;同时获得了1株分泌脂肪酶的菌—假单胞菌(Pseudomonas)、2株分泌磷脂酶C的菌株—肠杆菌(Enterobacteriaceae)和嗜麦芽寡养单胞菌(Stenotrophomonas);且姜黄素光动力能够减少这些菌株的数量(P<0.05),从而减少外源酶的数量。因此,姜黄素光动力可以通过降低牡蛎内源酶活性、减少外源酶数量来减缓牡蛎脂质的氧化水解。
In this paper,the effects of curcumin-mediated photodynamic treatment(PDT)on lipid degradation-related enzymes in oysters were investigated,aimed to elucidate the mechanism of curcumin-mediated PDT delaying lipids oxidative and hydrolysis from the perspective of enzymes.By detecting the activity of endogenous enzymes,selecting the strains secreted enzyme,identifying strain used 16S rDNA,analyzing enzyme-producing strains used Illumina MiSeq high-throughput sequencing technology in fresh oysters.The results showed that curcumin-mediated PDT could reduce the activity of endogenous lipoxygenase(LOX),phospholipase A 1(PLA 1),phospholipase A 2(PLA 2),and phospholipase D(PLD).One strains secreted lipases(Pseudomonas),two strains secreted phospholipase C(Enterobacteriaceae and Stenotrophomonas)were selected.What’s more,curcumin-mediated PDT could reduce the number of those bacteria secreted enzyme(P<0.05),thus reducing the number of exogenous enzymes.Therefore,curcumin-mediated PDT could delay the oxidative and hydrolysis of lipids by reducing the activity of endogenous enzymes and the amount of exogenous enzymes in oysters.
作者
张旭
卢娜
李兆杰
薛勇
袁诗涵
薛长湖
唐庆娟
ZHANG Xu;LU Na;LI Zhao-jie;XUE Yong;YUAN Shi-han;XUE Chang-hu;TANG Qing-juan(College of Food Science and Technology,Ocean University of China,Qingdao 266000,China)
出处
《食品工业科技》
CAS
北大核心
2020年第24期1-6,12,共7页
Science and Technology of Food Industry
基金
国家自然科学基金(31871867)。
关键词
姜黄素光动力
牡蛎
内源酶
产酶菌株
curcumin-mediated photodynamic treatment
oyster
endogenous enzyme
enzyme producing strains
