摘要
目的:观察2型糖尿病(T2DM)伴慢性牙周炎(CP)患者外周血中T辅助细胞17(Th17)和调节性T细胞(Treg)及细胞因子水平及与牙周状态的关系。方法:流式细胞术检测健康人(Ⅰ组)21例,单纯T2DM(Ⅱ组)25例,T2DM伴轻度CP(Ⅲ组)22例以及T2DM伴中重度CP(Ⅳ组)20例患者外周血中Th17、Treg细胞比例,ELISA法检测血清中白介素-17(IL-17)、转化生长因子-β(TGF-β)浓度,牙周检查记录牙周袋深度(PD)、出血指数(BI)。结果:Ⅳ组Th17、Th17/Treg以及IL-17水平较Ⅰ组和Ⅱ组更高(P<0.05),Th17、Th17/Treg、IL-17与PD、BI均呈正相关(P<0.05)。结论:Th17/Treg失衡可能在T2DM伴CP的发生发展中发挥重要作用。
Objective:To study the clinical significance of T helper cells 17(Th17 cells)and regulatory T cells(Treg cells)imbalance in patients with diabetes mellitus Type II(T2 DM)and chronic periodontitis(CP).Methods:The levels of Th17 and Treg in peripheral blood in 21 healthy controls(Group I),25 patients with simple T2 DM(GroupⅡ),22 patients with T2 DM and mild CP(Group III)and 20 patients with T2 DM and moderate to severe CP(Group IV)were measured by flow cytometry.The level of IL-17 and TGF-βin serum was measured by ELISA.The probing depth(PD)and bleeding index(BI)of all subjects were measured.Results:The levels of Th17,Th17/Treg and IL-17 of groupⅣwere higher than those of groupⅠandⅡ(P<0.05).There was a positive correlation between Th17,Th17/Treg ratio,IL-17 and respectively with PD and BI(P<0.05).Conclusion:Th17/Treg imbalance may play an important role in the development of T2 DM with CP.
作者
王佳
宋向欣
苏旭
陈晓涛
WANG Jia;SONG Xiangxin;SU Xu;CHEN Xiaotao(Shihezi University School of Medicine,832000,China;Department of Endocrinology,the People’s Hospital of Xinjiang Uygur Autonomous Region;Department of Oral Medicine,the People’s Hospital of Xinjiang Uygur Autonomous Region)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2020年第6期886-890,共5页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:81660185)
新疆维吾尔自治区自然科学基金(编号:2016D01C100)
乌鲁木齐市科学技术计划(编号:G161310006)。
