摘要
目的探究喉鳞癌组织中miR-1225-5p基因和细胞分裂周期蛋白14B(CDC14B)蛋白表达与临床病理特征及预后的关系。方法选取2012年1月~2015年6月在延安市人民医院行手术切除的67例喉鳞癌患者癌组织及其对应癌旁正常组织进行实验。采用qRT-PCR法检测癌组织及癌旁正常组织中miR-1225-5p mRNA表达;采用SP免疫组化染色法检测癌组织及癌旁正常组织中CDC14B蛋白表达;探究miR-1225-5p及CDC14B与临床病理特征及预后的关系。结果喉鳞癌组织中miR-1225-5p mRNA表达水平显著低于癌旁正常组织(1.7±0.5 vs 5.9±1.6),差异具有统计学意义(t=20.508,P<0.001);CDC14B蛋白阳性表达率显著高于癌旁正常组织(76.1%vs 37.3%),差异具有统计学意义(χ2=20.550,P<0.001)。miR-1225-5p mRNA不同表达在喉鳞癌患者分化程度(χ2=3.824,P=0.000)、T分期(χ2=1.859,P=0.034)、TNM分期(χ2=3.597,P=0.000)及是否淋巴结转移(χ2=2.191,P=0.016)之间的差异均具有统计学意义。CDC14B蛋白不同表达在喉鳞癌患者肿瘤直径(χ2=5.060,P=0.025)、分化程度(χ2=6.125,P=0.013)、T分期(χ2=10.129,P=0.001)、TNM分期(χ2=8.114,P=0.014)及是否淋巴结转移(χ2=4.273,P=0.038)之间的差异均具有统计学意义。miR-1225-5p低表达、CDC14B阳性患者术后5年总生存率低于miR-1225-5p高表达、CDC14B阴性患者(Log-rank P=0.046,0.023)。结论喉鳞癌组织中miR-1225-5p低表达、CDC14B阳性表达与喉鳞癌恶性程度及预后密切相关,可作为喉鳞癌潜在的肿瘤标志物及治疗靶点。
Objective To investigate the relationship between miR-1225-5p gene and CDC14B protein expression in laryngeal squamous cell carcinoma,clinicopathological features and prognosis.Methods A total of 67 patients with laryngeal squamous cell carcinoma who underwent surgical resection in Yan’an People’s Hospital from January 2012 to June 2015 were selected for the experiment.The expression of miR-1225-5p mRNA in cancer tissues and adjacent normal tissues was detected by qRT-PCR.SP immunohistochemical staining was used to detect CDC14B protein expression in cancer tissues and adjacent normal tissues,and explore the relationship between miR-1225-5P and CDC14B and the clinicopathological features and prognosis of the patients.Results The expression level of miR-1225-5p mRNA in laryngeal squamous cell carcinoma tissues was significantly lower than that in adjacent normal tissues(1.7±0.5 vs 5.9±1.6),with statistically significant difference(t=20.508,P<0.001).The positive expression rate of CDC14B protein was significantly higher than that of adjacent normal tissues(76.1%vs 37.3%),and the difference was statistically significant(χ2=20.550,P<0.001).The differences of miR-1225-5p mRNA expression in differentiation degree(χ2=3.824,P=0.000),T stage(χ2=1.859,P=0.034),TNM stage(χ2=3.597,P=0.000)and lymph node metastasis(χ2=2.191,P=0.016)were statistically significant.The differences of CDC14B expression in tumor diameter(χ2=5.060,P=0.025),differentiation degree(χ2=6.125,P=0.013),T stage(χ2=10.129,P=0.001),TNM stage(χ2=8.114,P=0.014)and lymph node metastasis(χ2=4.273,P=0.038)were statistically significant.The 5-year postoperative overall survival rate of patients with low miR-1225-5p expression and positive CDC14B expression was lower than that of patients with high miR-1225-5p expression and negative CDC14B expression(log-rank P value was 0.046,0.023).Conclusion The low expression of miR-1225-5p and the positive expression of CDC14B in laryngeal squamous cell carcinoma were closely related to the degree of malignancy and prognosis of laryngeal squamous cell carcinoma,which can be used as potential tumor markers and therapeutic targets for laryngeal squamous cell carcinoma.
作者
闫娟
安丽
张杰
拓明祥
YAN Juan;AN Li;ZHANG Jie;TUO Ming-xiang(Yan’an People’s Hospital,Shaanxi Yan’an 716000,China)
出处
《现代检验医学杂志》
CAS
2020年第6期114-118,共5页
Journal of Modern Laboratory Medicine
