摘要
近年来,同位素标记相对和绝对定量(Isobaric tags for relative and absolute quantification,iTRAQ)技术已成为蛋白质组学定量研究中一种强有力的工具,尤其在肿瘤相关领域备受关注,其突出优势为测定蛋白范围广泛、分析结果可靠、精确度高、重复性好等。大量文献也表明,iTRAQ技术在消化道肿瘤,如食管癌、胃癌、肝癌等肿瘤标志物筛查研究、肿瘤发生发展机制研究以及肿瘤治疗预后探索等方面得到广泛应用。在大规模定量生物学时代,iTRAQ技术在分子水平上深入了解消化道肿瘤的相关机制研究中越来越不可或缺。因此本文对iTRAQ技术在消化道肿瘤中的研究进展进行简要综述。
In recent years,the isotope-labeled relative and absolute quantification(Isobaric tags for relative and absolute quantification,iTRAQ)technology has become a powerful tool for the quantitative research of proteomics,especially in tumor-related fields.The advantages are a wide range of protein determination,reliable analytical results,high accuracy and good repeatability.A large number of literatures have also shown that iTRAQ technology has been widely used in gastrointestinal tumors,such as esophageal cancer,gastric cancer,liver cancer and other tumor marker screening research,the mechanism of tumor development,and the exploration of tumor treatment and prognosis.In the era of large-scale quantitative biology,iTRAQ technology is increasingly indispensable in the molecular level of in-depth understanding of the relevant mechanisms of digestive tract tumors.Therefore,this article briefly reviews the research progress of iTRAQ technology in gastrointestinal tumors.
作者
陈敏
刘莉萍(综述)
张玲(审校)
CHEN Min;LIU Liping;ZHANG Ling(Department of Gastroenterology,Inner Mongolia Medical University Baotou Clinical Medical College Baotou Central Hospital,Baotou 014040,China;General Internal Medicine,Jinchang Central Hospital,Gansu Province;Department of Gastroenterology,Baotou Central Hospital,Inner Mongolia Autonomous Region)
出处
《实用肿瘤学杂志》
CAS
2020年第6期549-553,共5页
Practical Oncology Journal
基金
内蒙古医科大学科技百万工程联合项目(编号:YKD2018KJBW(LH)083)。
