摘要
樟子松和黑皮油松具有抗病虫害、防风固沙能力强等特点,并具有较高的观赏性,成为城市森林中的主要树种。土壤微生物在维持森林植物土壤肥力方面起到重要作用。为探究樟子松和黑皮油松两种针叶人工林土壤细菌群落多样性和群落结构特征,采用高通量测序技术分析樟子松和黑皮油松人工林土壤细菌群落多样性指数、丰富度指数以及群落结构。结果表明,与樟子松人工林相比,黑皮油松人工林土壤细菌群落的Chao指数、ACE指数和Shannon指数更高。樟子松和黑皮油松人工林土壤具有相同的细菌门,主要优势门均为Proteobacteria、Acidobacteria和Actinobacteria。但樟子松人工林和黑皮油松人工林土壤细菌属却存在较大差异,RB41、Sphingomonas、norank-p--Saccharibacteria和norank-f-DA101-soil-group是导致樟子松和黑皮油松人工林差异的关键属。
Pinus sylvestris and Pinus tabulaeformis plantation,which resist to diseases and pests,prevent sand and wind and have high ornamental value,have become the main tree species in urban forests.Soil microorganisms play an important role in maintaining soil fertility of forest plants.The diversity and structural characteristics of soil bacterial community in Pinus sylvestris and Pinus tabulaeformis plantations are investigated to reveal the relationship between the growth of plantations and soil bacterial microbial community by using MiSeq sequencing of bacterial 16S rRNA gene to use analyze diversity index,diversity index and community structure.The results show that Chao index,ACE index and Shannon index in soil bacterial community of Pinus tabulaeformis plantation are higher than that in Pinus sylvestris plantation.There are the same bacterial phylum and the main dominant phylum in the soil of Pinus sylvestris and Pinus tabulaeformis plantation,including Proteobacteria,Acidobacteria and Actinobacteria,respectively.There are great differences in soil bacteria genera between Pinus sylvestris and Pinus tabulaeformis plantations.RB41,Sphingomonas,norank-p-Saccharibacteria,norank-f-DA101-soil-group are the key genus of the differences between Pinus sylvestris and Pinus tabulaeformis plantation.
作者
徐颖
庾嘉欣
于淼
孙广玉
Xu Ying;Yu Jiaxin;Yu Miao;Sun Guangyu(College of Life Science, Northeast Forest University, Harbin 150040, China)
出处
《黑龙江科学》
2020年第24期1-5,8,共6页
Heilongjiang Science
基金
东北林业大学大学生创新训练项目“利用高通量测序对4种不同树种人工林细菌和真菌群落结构的研究”(201910225355)。
关键词
樟子松
黑皮油松
人工林
细菌
群落结构
Pinus sylvestris
Pinus tabulaeformis
Man-made forests
Bacteria
Community structure
