摘要
目的研究早期持续暴露条件下邻苯二甲酸二丁酯(DBP)对秀丽线虫的毒性效应。方法设置不同剂量的染毒组(10μg/mL、50μg/mL、100μg/mL、200μg/mL)和对照组,将秀丽线虫的虫卵分别暴露到不同剂量的DBP染毒液中,染毒至成虫,分别考查其对线虫的身长、世代时间、后代数目、过氧化氢酶(CAT)活力和寿命的影响。结果与对照组相比,200μg/mL剂量组在线虫的身长、世代时间、后代数目、CAT活力和寿命指标上均存在显著抑制(P<0.05)。50μg/mL剂量组在后代数目、CAT活力两个指标上与对照组相比,存在显著的激活(P<0.05),显示出毒物的兴奋效应。结论DBP对秀丽线虫有明显的毒效应,可影响秀丽线虫的生长发育及生殖功能,诱导其活性氧产生,进而减少线虫的寿命。
Objective To investigate the toxic effect of dibutyl phthalate(DBP)in C.elegans by early continuous exposure.Methods The eggs of C.elegans were exposed to the different concentrations of DBP in low dose group(10μg/mL,50μg/mL),middle dose group(100μg/mL),high dose group(200μg/mL)and solvent control group,respectively.Results Compared to the solvent control,there were significant inhibitions in body length,generation time,brood size,activities of CAT and nematode longevity of the high dose group(P<0.05).There were significant activations in brood size,activities of CAT of the low dose group(50μg/mL)compared with the control group(P<0.05).Conclusion DBP had a significant toxic effect on C.elegans,which can affect the growth and reproductive function of induce the oxidative damage in C.elegans,then reduce the life span of C.elegans.
作者
杨晶
陈晓迪
高扬丽
赵云利
甄泉
YANG Jing(School of Public Health,Bengbu Medical College,Bengbu 233000,China)
出处
《牡丹江医学院学报》
2020年第6期4-7,共4页
Journal of Mudanjiang Medical University
基金
国家级大学生创新创业训练计划项目(201910367024)
安徽省教育厅重点项目(KJ2018A1021)。
