摘要
背景:在体育科研中,尿蛋白是评价运动负荷的一项重要指标。尿液蛋白质组学技术突破了以往的技术限制,所分析的差异蛋白图谱对于揭示机体对运动应激的适应机制和筛选表征运动水平的尿蛋白生物标志物具有重要作用。目的:应用功能内稳态概念概述正常尿液蛋白质组学研究历程及其在体育科研中评估机体病理性变化的最新研究进展,以期为运动负荷评定寻找无创方法提供参考。方法:以"urinary proteome,exercise,biomarkers,function-specific homeostasis;尿液,蛋白质组学,运动,生物标志物,功能内稳态"为关键词,在CNKI、万方、PubMed数据库及SCI数据库中检索1988年1月至2020年7月的相关文献并进行归纳分析。结果与结论:国内外在运动性蛋白尿方面进行诸多研究,主要集中在运用尿蛋白评定运动负荷大小、运动强度和身体机能。研究发现,运动后尿总蛋白、白蛋白、β2-微球蛋白显著增加,且具有明显的强度依赖。尿液蛋白质组学图谱的差异性表达可用于监测运动性疲劳、预防肌损伤、运动选材及机体病理性变化。
BACKGROUND:Urine protein may be an important indicator of the exercise load.Urinary proteomics technology has broken the technical limitations in sports science.It plays an important role in the analysis of differential protein profiles,revealing the body adaptation to exercise stresses,and screening the biomarkers of exercise performance.OBJECTIVE:To summarize the latest progress in normal urine proteomics and its application in sports science based on function-special homeostasis,aiming to seek a noninvasive method for exercise load assessment.METHODS:Using the terms"urinary proteome,exercise,biomarkers,function-specific homeostasis"as key words,CNKI,WanFang,PubMed and SCI databases were retrieved for relevant literatures published from January 1988 to July 2020.Eligible literatures were analyzed and summarized.RESULTS AND CONCLUSION:There are numerous studies on exercise-related urine proteins,which mainly focus on the assessment of exercise load,exercise intensity and somatic function based on urine protein determination.Urinary total protein,Alb,andβ2 microglobulin have a dramatic increase in a dosedependent manner after exercise.The differential protein expressions of urinary proteomics can be used as potential targets for monitoring exercise-induced fatigue,preventing muscle injury,selecting exercise instruments,and monitoring pathological changes of the body.
作者
蔺海旗
陈亮
唐璐
翁锡全
林文弢
Lin Haiqi;Chen Liang;Tang Lu;Weng Xiquan;Lin Wentao(School of Sports,South China University of Technology,Guangzhou 510641,Guangdong Province,China;School of Sports and Health,Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong Province,China;School of Sports,South China Normal University,Guangzhou 510006,Guangdong Province,China;Guangzhou Sport University,Guangzhou 510500,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第20期3259-3266,共8页
Chinese Journal of Tissue Engineering Research
基金
教育部人文社会科学研究青年基金项目(20YJCZH090),项目负责人:蔺海旗
广东省哲学社会科学“十三五”规划项目(GD19YTY01),项目负责人:蔺海旗
广东省教育科学“十三五”规划项目(2018GXJK001),项目负责人:蔺海旗。
关键词
尿液
蛋白质组学
生物标志物
功能内稳态
反兴奋剂
亚健康
负荷评定
差异表达
urine
proteomics
biomarkers
function-specific homeostasis
anti-doping
sub-healthy
exercise load assessment
differential expression
