摘要
为研究苜蓿连作栽培对土壤养分质量及根际微生物群落结构的影响,采用高通量测序技术,以4年生苜蓿根际土壤为研究对象,对其中的细菌及真菌群落结构进行了研究,同时分析了不同环境因子对微生物群落变化的贡献,为苜蓿改良土壤生态环境提供理论依据。试验结果表明:种植苜蓿可以降低土壤pH值,提高土壤中有机质和碱解氮含量,增加幅度分别为54.33%和24.93%。土壤中酸杆菌门、放线菌门和芽单胞菌门等细菌相对丰度增加了0.26%~6.39%,变形菌门、拟杆菌门、厚壁菌门和硝化螺旋菌门细菌相对丰度降低了0.06%~7.63%。真菌门类中子囊菌门、新美鞭菌门、毛霉门、球囊菌门、壶菌门、油壶菌门和丝足虫门(Cercozoa)比例提高了4.00%~225.00%,担子菌门和Aphelidiomycota所占比例分别降低了14.45%和80.00%。与环境因子的相关性分析表明,土壤中影响土壤细菌群落变化的主要环境因子为有机质、碱解氮和速效钾。结合不同微生物群落功能特性可以得出,苜蓿连作可以提高土壤养分含量及改善生态环境,提高土壤抗逆能力,适宜在盐碱地区推广。
In order to explore the effects of alfalfa continuous cropping on soil nutrient quality and rhizosphere microbial community structure,the bacterial and fungal community structure in rhizosphere soil of four-year-old alfalfa was studied by high-throughput sequencing technology,and the contribution of different environmental factors to the change of micro-bial community was analyzed,which provided theoretical basis for improving soil ecological environment of alfalfa.The re-sults showed that compared with the control,alfalfa planting decreased the soil pH value and increased the content of organ-ic matter and available nitrogen by 54.33%and 24.93%,respectively.The relative abundance of Acidobacterium,Actino-mycetes and Bacillus in soil increased by 0.26%-6.39%,while the relative abundance of Proteus,Bacteroides,Scleren-chyma and Nitrifying spirochetes decreased by 0.06%-7.63%.The proportions of Neutrocystis,Neoflagella,Mucor,Bal-loon,Potamopsis,Oil Potamopsis and Coccozoa increased from 4.00%to 225.00%,while the proportions of Basidiomycota and Aphelidiomycota decreased by 14.45%and 80.00%,respectively.The correlation of environmental factors showed that the main environmental factors affecting the change of soil bacterial community were organic matter,alkali-hydrolyzed nitrogen and available potassium.Combined with the functional characteristics of microbial community,it can be concluded that alfalfa continuous cropping can improve soil nutrients,ecological environment and soil stress resistance,which is suit-able for planting in saline-alkali areas.
作者
刘震
徐玉鹏
黄伟
黄素芳
王秀领
赵忠祥
阎旭东
LIU Zhen;XU Yupeng;HUANG Wei;HUANG Sufang;WANG Xiuling;ZHAO Zhongxiang;YAN Xudong(Cangzhou Academy of Agrieulture and Forestry Science,Cangzhou,Heibei 061001,China;Cangzhou Science and Technology Innovation Service Center,Cangzhou,Heibei 061001,Chima)
出处
《作物研究》
2020年第6期557-562,567,共7页
Crop Research
基金
河北省重点研发计划项目(20327406D
19226437D)
河北省现代农业产业技术体系草业创新团队栽培与信息化管理技术岗位(HBCT2018160202)。
关键词
苜蓿
盐碱地
土壤微生物
群落结构
alfalfa
saline-alkali soil
soil microorganisms
community structure
