摘要
目的:建立苍术及其炮制品的HPLC指纹图谱,并对其所含的6个有效成分进行定量分析。方法:采用HPLC,Waters XTERRA C18色谱柱(250 mm×4.6 mm,5μm),流动相乙腈-0.8%磷酸溶液梯度洗脱,检测波长为220 nm(检测苍术酮、β-桉叶醇、苍术苷A和白术内酯Ⅲ)、270 nm(检测苍术素和苍术素醇),流速为0.8 ml·min-1,建立生苍术和麸炒苍术的HPLC指纹图谱,通过相似度评价、聚类分析对苍术炮制前后指纹图谱进行分析,并对苍术素、苍术素醇、苍术酮、β-桉叶醇、白术内酯Ⅲ、苍术苷A进行含量测定。结果:建立了苍术及其炮制品的指纹图谱,以苍术素为参照峰,生苍术标定了21个共有峰,麸炒苍术标定了20个共有峰,与各自对照指纹图谱的相似度均大于0.955。苍术素、苍术素醇、苍术酮、β-桉叶醇、白术内酯Ⅲ、苍术苷A在炮制前的质量分数分别为3.1573,0.6636,1.0262,1.0014,0.2427,0.2385 mg·g-1;经炮制后,上述6种成分中的苍术酮消失,苍术素、苍术素醇、β-桉叶醇、白术内酯Ⅲ、苍术苷A在麸炒苍术中的含量分别为2.7019,0.5392,0.8008,0.3135,0.3214 mg·g-1。结论:苍术的HPLC指纹图谱在炮制前后发生了明显变化,通过对比共有峰的保留时间和色谱图,指认出部分成分;并发现20号峰为苍术的特有峰,因此峰20可用于区别生苍术和麸炒苍术;6个有效成分含量在炮制前后均发生了一定程度的变化,样品中苍术素、苍术素醇、苍术酮、β-桉叶醇含量排序为苍术>麸炒苍术,样品中白术内酯Ⅲ和苍术苷A含量排序则为麸炒苍术>苍术。
Objective: To establish the HPLC fingerprint of Rhizoma Atractylodis and its processed products,and to quantitatively analyze six components in Rhizoma Atractylodis before and after processing. Methods: HPLC was employed with a Waters XTERRA C18 column( 250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-0.8% phosphoric acid aqueous solution with gradient elution,the detection wavelength was set at 220 nm for atractylodes,β-eudesmol,atractyloside A and atracylenolide Ⅲ,and 270 nm for atractylodin and atractylodinol,and the flow rate was 0.8 ml·min-1. HPLC fingerprints of raw product and processed products of Rhizoma Atractylodis were established. Similarity evaluation and cluster analysis were used to analyze the chromatographic data. At the same time,the contents of atractylodin,atractylodinol,atractylodes,β-eudesmol,atracylenolide Ⅲ and atractyloside A were determined.Results: The HPLC fingerprints of Rhizoma Atractylodis and its processed products were established,and taking atractylodin as the reference peak,21 and 20 common peaks were demarcated for raw product and its processing products,respectively,and the similarities of fingerprints were above 0.955. The content of the above six components in raw product was 3.157 3,0.663 6,1.026 2,1.001 4,0.242 7 and 0.238 5 mg·g-1,respectively. After processing,atractylodes disappeared,and the content of atractylodin,atractylodinol,β-eudesmol,atracylenolide Ⅲ and atractyloside A in the processed products turned into 2.701 9,0.539 2,0.800 8,0.313 5 and0.321 4 mg·g-1,respectively. Conclusion: The HPLC fingerprint of Rhizoma Atractylodis changes significantly before and after processing. Some components are identified by retention time and chromatogram of common peaks,and peak 20 is characteristic peak of raw product,and can be used to distinguish the difference between raw product and its processed products;the contents of 6 components change to a certain extent before and after processing,the content order of atractylodin,atractylodinol,atractylodes and β-eudesmol in samples is raw product > processed products,while the content order of atracylenolide Ⅲ and atractyloside A is processed products> raw product.
作者
杨栋
陈悦梅
吴晓磊
骆微
蔡维娜
Yang Dong;Chen Yuemei;Wu Xiaolei;Luo Wei;Cai Weina(Pharmacy Department of Hangzhou First People’s Hospital,Hangzhou 310006,China)
出处
《中国药师》
CAS
2020年第12期2398-2402,共5页
China Pharmacist
