摘要
抗冻蛋白(antifreeze proteins,AFPs)是一类抗冻物质。最初发现抗冻蛋白能够与体内冰晶结合,通过抑制冰晶生长使极地鱼类能够在低于冰点的温度条件下生存。作为一种潜在的冷冻保护剂,AFPs已被用于低温保存生殖细胞和胚胎等生物样品。本文总结了近10年间AFPs在家畜组织和细胞冷冻保存技术方面的应用研究进展,探讨了AFPs在不同生物模型中抗冻保护作用的特性和原理,分析了AFPs在冷冻保存生物样本中可能产生影响的因素。同时,概述了AFPs对精子冷冻损伤的保护作用及其机制。总之,AFPs在冷冻保存中所表现出的无毒性和特有的生物学特性为家畜精液冷冻保存提供了一种新的解决方案,研究AFPs对精子的冷冻保护具有广阔的前景。
Antifreeze proteins(AFPs),a class of antifreeze substances,were initially found to bind to ice crystals in vivo,thereby contributing to inhibition of the growth of ice crystals that enabled polar fish to survive at the temperatures below freezing point.As a potential cryoprotectant,AFPs had been used for cryopreservation of germ cells,embryos and other biological samples.In this review,the application of AFPs to the cryopreservation of livestock tissues and cells in recent 10 years was summarized.The characteristics and principles of frost protection of AFPs under different biological models were discussed,and the possible influencing factors of AFPs in the cryopreservation of biological samples were analyzed.The protective effects of AFPs on sperm cryopreservation and its action mechanism were summarized,and the theoretical references were provided for the application of AFPs in cryopreservation of animal sperm.The non-toxicity and peculiar biological characteristics of AFPs in cryopreservation provided a new solution for livestock semen cryopreservation.Therefore,AFPs have a broad prospect in cryopreservation of sperm.
作者
衡诺
郭勇
陈余
王梁
盛熙晖
王相国
邢凯
倪和民
齐晓龙
HENG Nuo;GUO Yong;CHEN Yu;WANG Liang;SHENG Xihui;WANG Xiangguo;XING Kai;NI Hemin;QI Xiaolong(College of Animal Science and Technology,Beijing University of Agriculture,Beijing 102206,China;Beijing General Station of Animal Husbandry,Beijing 100107,China)
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2020年第6期660-666,共7页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
现代农业产业技术体系北京市家禽创新团队项目(BAIC04-2020)
“十三五”国家重点研发计划(2016YFD0700201)
2020年农业科技项目-科技创新服务能力建设-农业科技新星计划(20200210)
北京市农委“菜篮子”新型生产经营主体科技能力提升工程(2018)。
关键词
抗冻蛋白
抑制冰再结晶
热滞效应
冷冻保护剂
antifreeze protein
ice recrystallization inhibition
thermal hysteresis effect
antifreeze protectant
