摘要
目的:研究不同含量ZnO取代Al_2O_3对多孔生物玻璃(Bioglass,BG)支架的孔隙率、抗压强度(Compressive Strength,CS)、抗弯强度(Bending Strength,BS)、降解性能及体外矿化活性的影响,为开展新型高强度BG骨修复支架材料的研究和相关产品开发提供一定的理论支持。方法:本实验以铝硅酸盐玻璃为基础,用不同含量的ZnO取代Al_2O_3,采用熔融法制备基础玻璃,然后以聚氨酯海绵为模板,采用有机泡沫浸渍法制备多孔BG支架,分别为A组:不含ZnO的BG支架,B组:含1wt%ZnO的BG支架,C组:含2wt%ZnO的BG支架,D组:含3wt%ZnO的BG支架,并研究不同含量ZnO对多孔BG支架的孔隙率、CS、BS、降解性能及体外矿化活性的影响。孔隙率用阿基米德法测定,CS及BS用万能力学试验机测定,降解性能用多孔BG支架在模拟体液(Simulated Body Fluid,SBF)中的失重进行表征,表面形貌通过扫描电镜(Scanning Electron Microscope,SEM)观察,表面物象组成通过X线衍射分析(X-ray Diffraction,XRD)检测。结果:用不同含量的ZnO取代Al_2O_3后:①A、B、C、D四组孔隙率无统计学差异(P>0.05);②A、B、C、D四组CS逐渐下降,有统计学差异(P<0.05),两两比较,有统计学差异(P<0.05);③A、B、C、D四组BS逐渐下降,有统计学差异(P<0.05),两两比较,有统计学差异(P<0.05);④在SBF中浸泡7d后,A、B、C、D四组的失重比例逐渐增大,有统计学差异(P<0.05),两两比较,A组与B组无统计学差异(P>0.05),其余各组均有统计学差异(P<0.05);⑤在SBF中浸泡7d后,A组、B组无体外矿化活性,D组体外矿化活性优于C组。结论:在本实验中:①ZnO取代Al_2O_3对多孔BG支架的孔隙率影响较小,孔隙率主要受聚氨酯海绵尺寸的影响。②ZnO取代Al_2O_3能增强多孔BG支架的降解性能及体外矿化活性,但是会降低其抗压强度及抗弯强度。
Objective:The aim of this study was to investigate the effects of different percentage of ZnO instead of Al2O3 on the porosity,compressive strength(CS),bending strength(BS),degradation performance and mineralization activity in vitro of porous bioglass(BG)scaffolds.Subsequently,it can also provide some theoretical support for the research of new high strength BG bone repair scaffolds and the development of related products.Methods:This experiment is based on aluminosilicate glasses.Al2O3 in aluminosilicate glasses was replaced by ZnO with different percentage.The basic glass was prepared by melting method.Then,polyurethane foam was used as template and organic foam impregnation method was used to prepare porous BG scaffolds.Group A:BG scaffolds without ZnO;group B:BG scaffolds containing 1wt%ZnO;group C:BG scaffolds containing 2wt%ZnO;group D:BG scaffolds containing 3wt%ZnO.The effects of different percentage of ZnO on the porosity,CS,BS,degradation performance,and mineralization activity in vitro of porous BG scaffolds were studied.The porosity was determined by Archimedes method.The CS and BS were measured by Universal Capability Testing Machine.The degradation performance was characterized by weightlessness of porous BG scaffolds in simulated body fluids(SBF).Observing the surface morphology with the scanning electron microscope(SEM).Then we analyzed the surface image composition with the X-ray diffraction(XRD).Results:When Al2O3 was replaced with different percentage of ZnO:The porosity of group A,B,C and D had no statistical difference(P>0.05).The CS of group A,B,C and D decreased gradually with statistical difference(P<0.05),and there was statistical differences between each groups(P<0.05).The BS of group A,B,C and D decreased gradually with statistical difference(P<0.05),and there was statistical difference between each groups(P<0.05).After 7 days of immersion in SBF,the mass loss ratio of group A,B,C and D increased gradually with statistical difference(P<0.05),and there was no statistical difference between group A and group B(P>0.05),and the rest groups had statistical difference(P<0.05).After 7 days of immersion in SBF,group A and B exerted no mineralization activity in vitro,while group D exerted better mineralization activity in vitro than group C.Conclusion:In this experiment,the effect of using ZnO instead of Al2O3 on the porosity of porous BG scaffolds is little,and the porosity is mainly affected by the size of polyurethane sponge.Using ZnO instead of Al2O3 can enhance the degradation performance and mineralization activity of porous BG scaffolds in vitro,but reduce their compressive strength and bending strength.
作者
王薇
苏欣
王珍
王建春
王宏远
WANG Wei;SU Xin;WANG Zhen;WANG Jian-chun;WANG Hong-yuan(Fourth Affiliated Hospital of Inner Mongolia Medcal University,Inner Mongolia 014032,China)
出处
《口腔颌面修复学杂志》
2020年第6期326-332,348,共8页
Chinese Journal of Prosthodontics
基金
包头市卫生基金(项目编号:Wsjj2017094)。
