摘要
目的:建立根用芥菜子叶和下胚轴离体再生体系。方法:以根用芥菜无菌苗的子叶和下胚轴为外植体,比较不同浓度植物生长调节剂组合和不同光照条件培养对不定芽诱导率的影响,同时筛选不定芽增殖和生根的最佳培养基配方。结果:根用芥菜子叶和下胚轴最适不定芽分化培养基为MS+(5.0 mg/L 6-BA)+(0.5 mg/L NAA+3 mg/L AgNO 3),1000~1500 lx光照强度预培养可有效提高下胚轴的不定芽诱导率;不定芽的增殖效果以MS+(1.0 mg/L 6-BA)+(0.2 mg/L IAA)培养基最佳,生根效果以MS+0.2 mg/L IBA培养基最佳且移栽成活率达100%。结论:通过筛选不定芽分化、增殖和生根的适宜培养基及光照培养时间,建立了根用芥菜子叶和下胚轴离体再生体系,并获得了再生植株。
Objective:To establish the in vitro regeneration system of cotyledon and hypocotyl of Brassica juncea.Methods:The effects of different concentrations of plant growth regulators and different illumination conditions on adventitious bud induction rate were studied by using cotyledon and hypocotyl as explants of sterile mustard seedlings,meanwhile,the optimum media component for adventitious buds proliferation and rooting was selected.Results:MS+5.0 mg/L 6-BA+0.5 mg/L NAA+3 mg/L AgNO 3 was the optimal culture medium for adventitious buds differentiation of cotyledon and hypocotyl;the adventitious buds induction rate of hypocotyl can be effectively increased by preculture with 1000-1500 lx ray intensity.MS+1.0 mg/L 6-BA+0.2 mg/L IAA medium was the best medium for the proliferation of adventitious buds,MS+0.2 mg/L IBA medium was the most favorable for rooting and the survival rate was 100%.Conclusion:Through the optimum culture mediu selection of differentiation,proliferation and rooting of adventitious bud,and light culture time,the cotyledon and hypocotyl regeneration system of Brassica juncea was established,and the regenerated plants were obtained.
作者
周玉丽
顾安乐
李媛
许涛
钱海珍
杨磊
舒英杰
ZHOU Yuli;GU Anle;LI Yuan;XU Tao;QIAN Haizhen;YANG Lei;SHU Yingjie(College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China;Anhui Ruicheng Futian Agricultural Science and Technology Co.Ltd.,Hefei 230000,China;Anhui Wanbei Hometown Agricultural Technology Co.Ltd.,Linquan 236400,China)
出处
《安徽科技学院学报》
2020年第5期23-29,共7页
Journal of Anhui Science and Technology University
基金
安徽科技学院蔬菜学重点建设学科(AKZDXK2015C05)。
关键词
根用芥菜
子叶
下胚轴
不定芽诱导
离体再生
Brassica juncea
Cotyledon
Hypocotyl
Adventitious bud induction
Regeneration in vitro
