摘要
目的:建立人血浆中雷美替胺的气相色谱-质谱/质谱联用法(GC-MS/MS)检测方法,为临床药物分析提供依据。方法:血浆样品以乙酸乙酯提取后,经色谱柱BR-5MS毛细管柱(30 m×0.25 mm,0.25μm),程序升温,以多反应监测(MRM)模式进行离子检测。利用保留时间和离子对比例定性,外标法和工作曲线定量,建立人血浆中雷美替胺的GC-MS/MS检测方法。结果:雷美替胺标准曲线范围为1.0~1000.0 ng·mL^-1,定量下限为3.0 ng·mL^-1,日内、日间精密度均小于10%。结论:该方法灵敏准确,简便快速,适用于雷美替胺血药浓度检测。
OBJECTIVE To establish a gas chromatography-mass spectrometry/mass spectrometry(GC-MS/MS)method for the detection of ramelteon in human plasma and provide the basis for clinical drug analysis.METHODS After extraction with ethyl acetate,plasma samples were subjected to column BR-5 MS capillary column(30 m×0.25 mm,0.25μm)with temperature programming and ion detection in multiple reaction monitoring(MRM)mode.The quantitation determination of Remelteon was based on retention time and ration of ions.The quantitative analysis was base on external standard method and calibration curve.RESULTS The linear range of ramelteon was 1.0~1000.0 ng·mL^-1,the lower limit of quantification was 3.0 ng·mL^-1,and the RSD of inter and intra-day were both less than 10%.CONCLUSION The method is sensitive,accurate,simple and rapid,and suitable for the detection of remelton plasma concentration.
作者
王玲
王玉江
周青
孙世芃
孙爱丽
徐水英
赵玉婧
李欣
孙成文
WANG Ling;WANG Yu-jiang;ZHOU Qing;SUN Shi-peng;SUN Ai-li;XU Shui-ying;ZHAO Yu-jing;LI Xin;SUN Cheng-wen(Lab of Toxicant Analysis,the Fifth Medical Center of the People’s Liberation Army General Hospital,Beijing 100071,China;Beijing Fengtai District Center for Disease Control and Prevention,Beijing 100070,China;High-tech Borui Quality Inspection Center,Beijing 100021,China)
出处
《中国医院药学杂志》
CAS
北大核心
2020年第22期2322-2326,共5页
Chinese Journal of Hospital Pharmacy
关键词
雷美替胺
气相色谱-质谱/质谱
血药浓度
Ramelteon
gas chromatography-mass spectrometry/mass spectrometry
plasma concentration
