摘要
目的使用急-慢应激法构建腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,IBS-D)动物模型并评价其效果。方法选择20只SPF级Wistar大鼠为实验动物。采用随机数字法将其分为模型组和对照组,每组10只。模型组大鼠接受28 d慢性应激+1 d急性应激,对照组大鼠正常饲养,不接受任何干预及处理。于造模前后记录两组大鼠体质量、AWR疼痛压力阈值、蔗糖水摄入量,造模后记录两组稀便率、1 h排便量,将肠道组织标本行苏木精-伊红染色(Hematoxylin-Eosin staining,HE),观察肠道组织黏膜的微观结构。结果所有大鼠均顺利完成实验,无死亡发生。造模后,模型组大鼠1 h排便量(7.14±0.84)粒或次/h明显高于对照组(0.82±0.42)粒或次/h,差异有显著统计学意义(t=21.281,P=0.000);模型组大鼠湿便率(12.15±0.85)%明显高于对照组(0),差异有统计学意义(t=45.202,P=0.000);造模前,模型组与对照组大鼠疼痛压力阈值[(29.83±2.52)mmHg vs(30.00±2.71)mmHg]、蔗糖水摄入量[(10.00±1.45)ml vs(10.8±1.78)ml]及体质量[(203.4±8.73)g vs(202.85±7.14)g]比较,差异无统计学意义(P>0.05)。造模后,模型组大鼠疼痛压力阈值(27.17±1.50)mmHg、蔗糖水摄入量(14.1±1.81)ml及体质量(279.37±9.47)g明显低于对照组(38.67±2.33)mmHg、(19.4±1.26)ml及(313.91±6.66)g,差异有统计学意义(t=13.123,P=0.000;t=7.600,P=0.000;t=9.434,P=0.000)。两组大鼠结肠组织微观结构均未见黏膜明显水肿、糜烂、溃疡、出血。结论急-慢应激法构建大鼠模型在肠道症状、动力及感知、情绪障碍、微观结构方面与IBS-D发病特点一致,造模方法有效,为IBS-D的动物研究提供了一种方法。
Objective To construct an animal model of diarrhea-predominant irritable bowel syndrome(IBS-D)using acute-chronic stress method and evaluate its effect.Methods Twenty SPF Wistar rats were selected as experimental animals which were randomly divided into model group and control group by a random number method,with 10 rats in each group.Rats in model group received 28 days of chronic stress and 1 day of acute stress,and the rats in control group were reared normally without any intervention and treatment.The body weight,AWR pain pressure threshold,and sucrose water intake of the two groups were recorded before and after modeling,and the wet feces rate and defecation number of the two groups were recorded after modeling.Hematoxylin-Eosin(HE)staining was performed on intestinal tissue specimens to observe the microstructure of intestinal tissue mucosa.Results All rats successfully completed the experiment without death.After modeling,the defecation number of the rats in model group(7.14±0.84)granules or times/hour was significantly higher than that in control group(0.82±0.42)granules or times/hour,with a significant difference between two groups(t=21.281,P=0.000);wet feces rate in model group(12.15±0.85)%was significantly higher than those in control group(0),the difference was statistically significant(t=45.202,P=0.000);before modeling,there were no significant differences of threshold pressure of pain[(29.83±2.52)mmHg vs(30.00±2.71)mmHg]sucrose water intake[(10.00±1.45)ml vs(10.8±1.78)ml]and body weight[(203.4±8.73)g vs(202.85±7.14)g]between model group and control group(P>0.05).After modeling,pain pressure threshold[(27.17±1.50)mmHg vs(38.67±2.33)mmHg],sucrose water intake[(14.1±1.81)ml vs(19.4±1.26)ml],and body weight[(279.37±9.47)g vs(313.91±6.66)g]in model group were significantly lower than those in control group,the differences were statistically significant(t=13.123,P=0.000;t=7.600,P=0.000;t=9.434,P=0.000).There were no obvious edema,erosion,ulcers,or bleeding in the mucosa of the colonic tissues microstructure of the two groups.Conclusion The rat model of acute-chornic stress is consistent with the pathological characteristics of IBS-D in terms of intestinal symptoms,motility and perception,mood disorders,and microstructure.This modeling method is effective and can provide a way in IBS-D model research.
作者
贺星
刘卫
唐郡
朱斌
李超
郑岩
崔立红
HE Xing;LIU Wei;TANG Jun;ZHU Bin;LI Chao;ZHENG Yan;CUI Lihong(Department of Gastroenterology,the 901th Hospital of Joint Logistics Support Force of PLA,Hefei 230031;Department of Gastroenterology,the Sixth Medical Center of PLA General Hospital,China)
出处
《胃肠病学和肝病学杂志》
CAS
2020年第12期1386-1390,共5页
Chinese Journal of Gastroenterology and Hepatology
基金
国家自然科学基金面上项目(81670494)。
关键词
腹泻型肠易激综合征
动物模型
急-慢应激
大鼠
评价
Diarrhea-predominant irritable bowel syndrome
Animal model
Acute-chronic stress
Rats
Evaluation
