摘要
目的探讨血清miR-224-5p在PDAC中的表达及其对胰腺癌早期临床诊断的意义。方法选取青岛市市立医院2018年8月至2020年4月间收治的40例PDAC患者(11例早期PDAC,29例中晚期PDAC)、21例慢性胰腺炎患者和40名健康志愿者为研究对象,应用qRT-PCR方法检测各组血清miR-224-5p水平,分析其与临床病理参数之间的相关性。绘制miR-224-5p、CA19-9及miR-224-5p联合CA19-9检测的受试者工作特征曲线(ROC),计算诊断的灵敏度和特异度。结果早期PDAC组、中晚期PDAC组、慢性胰腺炎组和健康对照组血清miR-224-5p水平分别为3.21(2.01,4.60)、4.70(3.50,8.26)、1.72(1.02,2.78)、1.38(0.89,2.11),中晚期PDAC组血清miR-224-5p水平显著高于早期PDAC组,早期PDAC组又显著高于慢性胰腺炎组和健康对照组,差异有统计学意义(P值均<0.05)。血清miR-224-5p联合CA19-9、miR-224-5p、CA19-9检测对PDAC的诊断灵敏度依次为95.0%、85.0%、67.5%,特异度依次70.0%、82.5%、87.5%;对早期PDAC的诊断灵敏度依次为90.9%、72.7%、63.6%,特异度依次为72.5%、85.0%、87.5%。血清miR-224-5p联合CA19-9诊断PDAC的灵敏度最高。PDAC患者血清miR-224-5p水平与肿瘤TNM分期、淋巴结转移和远处转移相关(P值均<0.05)。结论PDAC患者血清miR-224-5p表达水平明显上调,且与肿瘤TNM分期、淋巴结和远处转移密切相关。血清miR-224-5p及其与CA19-9联合诊断早期PDAC的灵敏度高于单纯CA19-9,可作为早期筛查PDAC潜在的较敏感的生物学标志物。
Objective To explore the expression of serum miR-224-5p in PDAC and its significance for early clinical diagnosis.Methods From August 2018 to April 2020,40 patients with PDAC(11 patients with early PDAC,29 patients with advanced PDAC),21 patients with chronic pancreatitis and 40 healthy volunteer controls admitted in Qingdao Municipal Hospital were enrolled.The level of serum miR-224-5p in each group was detected by qRT-PCR method,and the correlation with clinicopathological parameters was analyzed.The receiver-operating characteristic(ROC)curve of miR-224-5p,CA19-9 and miR-224-5p combined with CA19-9 were drawn,and the sensitivity and specificity of the diagnosis were calculated.Results The serum miR-224-5p levels in early PDAC group,middle and late PDAC group,chronic pancreatitis group and healthy control group were 3.21(2.01,4.60),4.70(3.50,8.26),1.72(1.02,2.78)and 1.38(0.89,2.11),respectively;and the level of serum miR-224-5p in the middle and late PDAC group was significantly higher than that in the early PDAC group,and that in the early PDAC group was significantly higher than that in the chronic pancreatitis group and the healthy control group,and all the differences were statistically significant(P<0.05).The sensitivity of serum miR-224-5p combined with CA19-9,miR-224-5p,and CA19-9 in the diagnosis of overall PDAC was 95.0%,85.0%and 67.5%,respectively;and the specificity was 70.0%,82.5%and 87.5%,respectively.The sensitivity for early PDAC was 90.9%,72.7%and 63.6%,and the specificity was 85.0%,72.5%and 87.5%,respectively.MiR-224-5p combined with CA19-9 has the highest specificity in the diagnosis of PDAC.The level of serum miR-224-5p in patients with PDAC was correlated with TNM stage,lymph node metastasis and distant metastasis(all P values<0.05).Conclusions The expression of serum miR-224-5p was significantly up-regulated in patients with early PDAC,and The level of serum miR-224-5p in patients with PDAC was correlated with TNM stage,lymph node metastasis and distant metastasis.The sensitity of serum miR-224-5p and miR-224-5p combined with CA19-9 for early PDAC diagnosis were superior to CA19-9 alone,which can be used as a potential sensitive biological marker for early screening of PDAC.
作者
贾孝娟
张巍巍
李文利
姜大磊
谢方瑜
张一
付来琳
王尧
陈斌
宋敏
李静
解祥军
Jia Xiaojuan;Zhang Weiwei;Li Wenli;Jiang Dalei;Xie Fangyu;Zhang Yi;Fu Lailin;Wang Yao;Chen Bin;Song Min;Li Jing;Xie Xiangjun(Department of Gastroenterology,Qingdao Municipal Hospital,Affiliated Hospital of Qingdao University,Qingdao 266011,China;Department of Cardiology,Qingdao Municipal Hospital,Affiliated Hospital of Qingdao University,Qingdao 266011,China;Hepatological Surgery Department,Qingdao Municipal Hospital,Affiliated Hospital of Qingdao University,Qingdao 266011,China;Endoscope Center,Qingdao Municipal Hospital,Affiliated Hospital of Qingdao University,Qingdao 266011,China)
出处
《中华胰腺病杂志》
CAS
2020年第6期412-417,共6页
Chinese Journal of Pancreatology
关键词
胰腺肿瘤
微RNAS
生物学标记
早期诊断
Pancreatic neoplasms
MicroRNAs
Biological markers
Early diagnosis
