摘要
通过高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定香蕉枯萎病感病品种‘巴西蕉’和抗病品种‘南天黄’根系分泌物中10种酚酸物质的含量,并选取含量具有明显差异的酚酸物质验证其对尖孢镰刀菌古巴专化型(Fusarium oxysporium f.sp.cubense,Foc)4号生理小种菌落生长的抑制效果。研究发现,接种Foc4诱导后,对羟基苯甲酸和肉桂酸仅在抗病品种‘南天黄’根系分泌物中检测到,邻苯二甲酸的含量在抗病品种中高于感病品种近2倍。在PDA培养基中邻苯二甲酸、对羟基苯甲酸和肉桂酸对Foc4菌落生长的抑制率随着含量的增加而显著增强。研究结果表明香蕉根系分泌物中酚酸物质与香蕉品种抗性之间存在密切的相关性。
The High-performance Liquid Chromatography(HPLC)was used to determine the content of 10 phenolic acids in root exudates of susceptible and resistant banana cultivars(Brazilian and Nantianhuang),and the phenolic acids with obvious content differences in different treatments were selected to verify its inhibitory effect on the growth of Foc4.The study found that after induction with Foc4,p-hydroxybenzoic acid and cinnamic acid were detected only in the root exudates of resistant cultivar,and the content of phthalic acid in disease resistant cultivar was nearly twice that disease susceptible cultivar,and the inhibition rate of phthalic acid,p-hydroxybenzoic acid and cinnamic acid on the growth of Foc4 increased significantly with increasing concentration.The result also showed that there was a close correlation between phenolic acids from banana root exudates and banana cultivar resistance,which provided a certain theoretical basis for the subsequent selection of banana Fusarium wilt resistant varieties.
作者
翟子翔
李得铭
邓涛
邓大豪
杨腊英
周游
郭立佳
黄俊生
ZHAI Zixiang;LI Deming;DENG Tao;DENG Dahao;YANG Laying;ZHOU You;GUO Lijia;HUANG Junsheng(College of Tropical Cros Hainan University,Haikou 570228,China;Institute of Environment and Plant Protection,Chinese Academy of Tropical Agricultural Science,Haikou 571101,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2020年第11期2207-2214,共8页
Acta Horticulturae Sinica
基金
国家重点研发专项项目(2017YFD0202100)
海南自然科学基金创新研究团队项目(2017YFD020060206)
2017年度中国热带农业科学院环境与植物保护研究所结余经费自主选题专项项目(hzsjy2017004)。
