摘要
目的研究miR-122在肾癌中的作用及其与Sprouty2的相互关系。方法使用Western blot检测4个肾癌组织和相邻正常组织中Sprouty2的表达水平,RT-qPCR检测42个肾癌组织和相邻正常组织中的miR-122表达水平。下调miR-122或敲低Sprouty2后通过CCK-8实验检测肾癌细胞增殖情况,并使用双荧光素酶基因实验明确miR-122和Sprouty2之间的关系。结果肾癌组织中Sprouty2表达水平低于正常组织(P<0.05),miR-122表达水平高于正常组织(P<0.05)。miR-122下调后肾癌细胞的增殖能力下降(P<0.05)。敲低Sprouty2表达水平能促进肾癌细胞的体外增殖(P<0.05)。miR-122抑制剂能与Sprouty23′-UTR结合,共转染后增强Sprouty2基因的活性。结论Sprouty2基因是miR-122的靶基因。miR-122可以通过抑制Sprouty2基因的活性,促进肾癌的发生、发展。
Objective The aim of this study is to determine the biological function of miR-122 in renal cell carcinoma(RCC)and identify new molecular targets regulated by miR-122.Methods The expression of Sprouty2 in 4 samples of RCCand adjacent non-tumor tissues were detected by Western blot.The levels of miR-122 in 42 primary RCC and adjacent non-malignant tissue samples were measured by reverse transcription polymerase chain reaction(RT-qPCR).CCK-8 wasused to assess the proliferation of cancer cells after miR-122 had been down-regulatedor Sprouty2 had been knocked down.The dual luciferase reporter assay was used to determine the relationship between miR-122 and Sprouty2.Results The expression level of Sprouty2 was lower in RCC tissue samples compared to adjacent normal tissues(P<0.05).The level of miR-122 was higher in primary RCC tissue samples compared to adjacent normal tissue samples(P<0.05).Down-regulation of miR-122 significantly impaired the proliferation of RCC cell lines in vitro(P<0.05).Sprouty2 knockdown promoted in vitro proliferation of RCC cell lines(P<0.05).Conclusions The Sprouty2 gene may be a direct target of miR-122.MiR-122 might promote proliferation,migration and invasion of RCC cells by suppressing the activity of Sprouty2.
作者
周海
潘凯
陈玉明
申余勇
周明
贺兴军
费尚春
王小祥
ZHOU Hai;PAN Kai;CHEN Yuming;SHEN Yuyong;ZHOU Ming;HE Xingjun;FEI Shangchun;WANG Xiaoxiang(Department of Urology, Affiliated Hospital of Yangzhou University, Yangzhou 225009, China)
出处
《中国肿瘤外科杂志》
CAS
2020年第6期518-523,共6页
Chinese Journal of Surgical Oncology
基金
扬州市重点研发计划(社会发展)(YZ2016082)。
