摘要
以碱提酸沉法制备茶树籽蛋白质,并用微波辅助酶解法制备茶树籽多肽。研究了胃蛋白酶用量、酶解温度、酶解时间、酶解液pH值等工艺因素对酶解产物的蛋白质水解度和多肽浓度的影响,并采用正交试验法优化微波辅助酶解的工艺条件。结果表明,产物的蛋白质水解度和多肽浓度随着加酶量提高和水解时间延长呈稳定上升后趋于平缓的趋势,而随着水解温度提高和酶解液pH值升高呈先上升后下降趋势,酶解液pH值对结果有显著影响。微波辅助酶解工艺可显著缩短蛋白质酶解进程、提高酶解液中多肽的含量,在胃蛋白酶用量6000 u/g、酶解温度50℃、酶解时间15 min、酶解液pH值3.5等的优化条件下,酶解产物的蛋白质水解度为13.65%、多肽浓度为13.18 g/L。
The seeds protein of Camellia sinensis was prepared by the alkali-extracting and acid precipitation method,and the polypeptide was prepared by the microwave-assisted enzymolysis technology.The effects of process factorsincluding enzyme dosage,temperature and time of enzymolysis as well as pH value of enzymolysis solution on the degree of protein hydrolysis and the polypeptide concentration were studied.The orthogonal method was adopted to optimize the hydrolysis process condition.The results showedthat,the degree of protein hydrolysis and the polypeptide concentration increased steadily first and then tended to be levelling off with the increaseof enzyme dosage and reaction time,while it increased first and then decreased with the increase of reaction temperature and pH value ofenzymolysis solution.The pH value ofenzymolysis solution had significant influence on the results.The protein enzymolysis process was significantly shortened and the content of polypeptide in enzymolysis solution was improved by the microwave-assisted enzymolysis technology.Under the optimized conditions ofthe pepsin dosage of 6000 u/g,reaction temperature of 50℃,reaction time of 15 min,the enzymolysissolution pH value of 3.5,the degree of protein hydrolysis was 13.65%,the polypeptide concentration was 13.18 g/L.
作者
郑德勇
陈成聪
颜阳蕾
张洁婷
叶乃兴
Zheng Deyong;Chen Cengcong;Yan Yanglei;Zhang Jieting;Ye Naixing(College of Material Engineering,Fujian Agriculture and Forestry University,Fuzhou 350002;National Engineering Research Center for Tea Quality and Safety,Quanzhou 362441;College of Horticulture,Fujian Agriculture and Forestry University,Key Laboratory of Tea Science in Universities in Fujian Province,Fuzhou 350002)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2020年第12期135-140,155,共7页
Journal of the Chinese Cereals and Oils Association
基金
国家茶叶质量安全工程技术研究中心开放课题基金(2017NTQS0402)。
关键词
茶树籽
蛋白质
微波
酶解
多肽
seeds of Camellia sinensis(L.)O.Ktze.
protein
microwave
enzymolysis
polypeptide
