摘要
以阿霉素(DOX)盐酸为模型药物,制备叶酸修饰的pH响应型长循环固体脂质纳米粒(DOX@SLN-PEOz-FA),进行制剂学性质、体外释药和体外抗肿瘤活性评价。实验制得的修饰纳米粒平均粒径为(245.46±47.28)nm,包封率为(92.46±5.30)%,体外释药表明纳米粒在pH=5.0下释药最快,细胞摄取实验结果表明经叶酸修饰后的载体可以有效靶向肿瘤细胞,体外抗肿瘤活性结果表明DOX@SLN-PEOz-FA对人乳腺癌细胞(MCF-7)生长的抑制作用最强。
The folic acid-modified pH-responsive long-circulating solid lipid nanoparticles(DOX@SLN-PEOz-FA)were prepared with doxorubicin(DOX)as a model drug.Their formulation properties,in vitro release and antitumor activity were evaluated.The results showed that average particle size of the modified nanoparticles was(245.46±47.28)nm,and the encapsulation efficiency was(92.46±5.30)%.The in vitro release results indicated that the drug released fastest at pH 5.0.The cell uptake assay results revealed that the carrier modified with folic acid could effectively target tumor cells.The anti-tumor activity in vitro showed that DOX@SLN-PEOz-FA had the strongest inhibitory effect on human breast cancer cells MCF-7 cell growth.
作者
王小宁
闫梦茹
梁晓燕
高迎春
马远涛
WANG Xiaoning;YAN Mengru;LIANG Xiaoyan;GAO Yingchun;MA Yuantao(College of Pharmacy,Xi’an Medical University,Xi’an 710021,China)
出处
《化工科技》
CAS
2020年第6期19-23,36,共6页
Science & Technology in Chemical Industry
基金
陕西省教育厅专项科研计划项目(19JK0757)
西安医学院青年科研基金项目(2018QN12)
西安医学院药物研究所开放基金项目(2016YXXK07)
陕西省缺血性心血管疾病重点实验室开放基金项目(2017ZDKF09)
中尼友好拉吉姆医学实验室开放基金项目(18LJM06)。
