摘要
为探索栽培种花生出仁率的遗传机制,以花育36号与6-13为亲本构建了包含181个家系的重组自交系(recombinant inbred line,RIL)群体,以该群体F2:9代为试验材料,测定了2个环境下的出仁率表型数据,利用3种方法(单环境分析、多环境联合分析和上位性互作分析)进行相关QTL定位。结果表明,在不同环境下RIL群体出仁率均表现为超亲遗传,广义遗传率为0.79。基于单环境QTL分析,共检测到6个相关位点qKP7、qKP8、qKP9、qKP13、qKP16和qKP18,分布于6个连锁群上,表型贡献率为4.20%~15.14%。其中qKP7和qKP9在两个环境下均稳定表达,且qKP7表型贡献率大于10%,为主效QTL。基于多环境联合分析,发现这6个QTL的加性效应对表型的总贡献率为38.57%,加性与环境互作效应对表型总贡献率为4.13%。上位性互作分析共检测到6对上位性QTL,共涉及10个位点,分别位于第3、7、11、12、15、16、17、18染色体,上位性互作对表型贡献率为2.90%~4.58%,上位性QTL与环境互作对表型的总贡献率为0.49%。本研究定位到6个主效应QTL和6对上位性QTL,为后续相关基因克隆和花生出仁率性状改良提供理论基础。
To explore the genetic basis modulating kernel percentage in peanut,a recombinant inbred line(RIL)population(181 lines)was constructed by crossing two parents Huayu36 and 6-13.The kernel percentage of RIL population(F2:9)were investigated under two environments(E1,E2),which displayed transgressive segregations with a broad-sense heritability of 0.79.QTL mapping analysis was performed using three methods,including individual environmental analysis,multi-environmental joint analysis and epistatic interaction analysis.Individual environmental analysis found 6 QTLs(qKP7,qKP8,qKP9,qKP13,qKP16 and qKP18)on 6 linkage groups with phenotypic variation explained(PVE)of 4.20%~15.14%.Among them,qKP7 and qKP9 could be detected in two environments,and qKP7 was considered as a major effect QTL accounting for PVE>10%.Multi-environmental joint analysis demonstrated that the collective PVE by additive effect was 38.57%,and interaction effect of additive×environment was 4.13%.Epistatic interaction analysis identified 6 pairs of epistatic QTLs involving 10 loci on chromosomes 3,7,11,12,15,16,17 and 18.The PVE by interaction of epistasis ranged from 2.90%to 4.58%,and the collective PVE by interaction of epistatic QTL×environment was 0.49%.These overall results provided a theoretical basis for both improvement of peanut kernel percentage and cloning of associated genes.
作者
张胜忠
胡晓辉
杨珍
徐胜
郭进涛
侯刚
张智猛
苗华荣
陈静
ZHANG Sheng-zhong;HU Xiao-hui;YANG Zhen;XU Sheng;GUO Jin-tao;HOU Gang;ZHANG Zhi-meng;MIAO Hua-rong;CHEN Jing(Shandong Peanut Research Institute,Qingdao 266100,China;Anhui General Station of Agricultural Technology Extension,Hefei 230001,China;Hezhuang Agricultural Service Center of Xinzheng,Zhengzhou 451150,China;Tieling Agricultural Research Institute,Tieling 112616,China)
出处
《花生学报》
北大核心
2020年第4期7-13,共7页
Journal of Peanut Science
基金
山东省农业良种工程(2017LZGC003,2017LNZ033)
山东省农业科学院创新工程(CXGC2016B02,CXGC2018E21)
青岛市科技惠民示范引导专项(20-3-4-26-nsh)。
关键词
花生
重组自交系
出仁率
数量性状位点
peanut
recombinant inbred line
kernel percentage
quantitative trait locus
