摘要
提出了一种基于纳米金粒子比色法的汞离子检测方法,采用铋试剂Ⅱ修饰的纳米金粒子胶体悬浮液检测汞离子。汞离子与铋试剂Ⅱ选择性配位,使纳米金粒子胶体悬浮液反团聚,汞离子浓度与溶液吸光度呈一定的线性关系,线性范围为0.05μg/L^1 mg/L,相对标准偏差为1.98%~3.52%。在实验条件下,采用SavitzkyGolay一阶导数对连续投影算法提取的特征波长进行了预处理,通过线性支持向量机回归模型进行建模与分析,该模型具有良好的自预测能力和实际预测能力。校正集的相关系数为0.9685,均方根误差为0.0412 mg/L,验证集的相关系数为0.9600,均方根误差为0.0434 mg/L。本文方法为提高汞离子浓度的检测效率提供了有效支持。
Mercury ion concentration is one of the key parameters affecting water quality environment,and a method for detecting mercury ion based on gold nanoparticle colorimetry was proposed.The gold nanoparticle colloidal suspension modified by bismuth reagentⅡwas used to detect mercury ion.The mercury ion selectively coordinated with bismuth reagentⅡ,which made the gold nanoparticle colloidal suspension anti agglomerate.There was a certain linear relationship between the concentration of mercury ion and the absorbance of the solution,the linear range was 0.05μg/L^1 mg/L,and the relative standard deviation was 1.98%~3.52%.Under the experimental conditions,the characteristic wavelengths extracted from successive projection algorithm were pretreated by SavitzkyGolay first derivative.Through linear support vector machine regression model modeling and analysis,the model had good self prediction ability and practical prediction ability.The correlation coefficient of the calibration set was 0.9685,the root mean square error of calibration set was 0.0412 mg/L,the correlation coefficient of the validation set was 0.9600,and the root mean square error of validation set was 0.0434 mg/L.Therefore,the method based on gold nanoparticle colorimetry can provide effective support for improving the detection efficiency of mercury ion concentration.
作者
郭昱辰
刘春红
叶荣珂
孔庆辰
张玉泉
GUO Yuchen;LIU Chunhong;YE Rongke;KONG Qingchen;ZHANG Yuquan(College of Information and Electrical Engineering,China Agricultural University,Beijing 100083,China;National Fishery Digital innovation Center,Beijing 100083,China)
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2020年第S02期382-387,共6页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家重点研发计划项目(2017YFE0122100)。
关键词
汞离子
纳米金粒子比色法
紫外可见光谱
连续投影算法
支持向量机回归
mercury ion
gold nanoparticle
ultraviolet visible spectroscopy
successive projection algorithm
linear support vector machine regression model
