摘要
目的:从肠道菌群-短链脂肪酸(SCFAs)探讨薯蓣粥对糖尿病合并脑卒中大鼠糖脂代谢的影响。方法:选择SPF级wistar大鼠,使用腹部注射STZ、Zea Longa线栓法制作糖尿病合并缺血性脑卒中模型,成模后随机分为模型组、薯蓣粥组;另取一组为假手术组(不做尼龙单丝插入,其余造模过程均相同)。模型组、假手术组予0.9%氯化钠注射液5 ml灌胃,薯蓣粥组以薯蓣粥5 ml灌胃,共4周。干预后每周检测大鼠空腹血糖(FBG),干预结束后检测大鼠血糖、血脂4项[三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、总胆固醇(TC)]、脂联素(ADNP)、SCFAs水平肠道菌群多样性及物种丰度。结果:TG、TC、LDL-C、HDL-C、ADNP、SCFAs各指标,假手术组、薯蓣粥组与模型组比较,差异均有统计学意义(P<0.05或P<0.01)。干预第3、4周薯蓣粥组与模型组FBG比较,差异均有统计学意义(P<0.05)。模型组的OUTs数量、Chaol指数、Shannon指数均低于假手术组,但Chaol指数、Shannou指数均高于薯蓣粥组,组间比较,差异均有统计学意义(P<0.05或P<0.01)。薯蓣粥组中,Clostridiales、Ruminococcaceae、Prevotella、Lachnos Piraceae等能够促进SCFAs产生的菌属较模型组增多。结论:薯蓣粥可通过调控肠道菌群,增加SCFAs,从而改善糖尿病合并脑卒中大鼠的糖脂代谢。
Objective:To investigate the effect of Dioscorea opposita Thunb.porridge on glycolipid metabolism in rats with diabetes and stroke from the aspect of gut microbiota-short-chain fatty acids(SCFAs).Methods:Specific pathogen-free Wistar rats were treated with intraperitoneally injected streptozotocin and the Zea Longa suture method to establish a model of diabetes with ischemic stroke,and after the model was successfully established,the rats were randomly divided into model group and Dioscorea opposita Thunb.porridge group;a sham-operation group was also established(treated with the same modeling process without the insertion of nylon monofilament).The rats in the model group and the sham-operation group were given 5 ml 0.9%sodium chloride injection by gavage,and those in the Dioscorea opposita Thunb.porridge group were given 5 m L Dioscorea opposita Thunb.porridge by gavage,for 4 weeks in total.Fasting blood glucose(FBG)was measured every week during intervention,and related indices were measured after the intervention ended,including blood glucose,four blood lipid parameters[triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),and total cholesterol(TC)],adiponectin(ADNP),levels of SCFAs,and diversity and species abundance of gut microbiota.Results:There were significant differences in TG,TC,LDL-C,HDL-C,ADNP,and SCFAs between the sham-operation group,the Dioscorea opposita Thunb.porridge group,and the model group(P<0.05 or P<0.01).At weeks 3 and 4 of intervention,there was a significant difference in FBG between the Dioscorea opposita Thunb.porridge group and the model group(P<0.05).The model group had significantly lower OUTs,Chaol index,and Shannon index than the sham-operation group and significantly higher Chaol index and Shannon index than the Dioscorea opposita Thunb.porridge group(P<0.05 or P<0.01).Compared with the model group,the Dioscorea opposita Thunb.porridge group had significant increases in Clostridiales,Ruminococcaceae,Prevotella,and Lachnospiraceae which promoted the production of SCFAs.Conclusion:Dioscorea opposita Thunb.porridge can improve glycolipid metabolism in rats with diabetes and stroke by regulating gut microbiota and increasing SCFAs.
作者
洪雪珮
庞书勤
罗宗婷
周建
陈芳
戴燕铃
李霞
张佳惠
HONG Xuepei;PANG Shuqin;LUO Zongting;ZHOU Jian;CHEN Fang;DAI Yanling;LI Xia;ZHANG Jiahui(Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian,China)
出处
《湖南中医杂志》
2020年第11期179-183,共5页
Hunan Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(81904269)
福建省康复产业研究院开放项目(2015Y2001-51)。
关键词
糖尿病合并脑卒中
薯蓣粥
糖脂代谢
肠道菌群
实验研究
diabetes and stroke
Dioscorea opposita Thunb.porridge
glycolipid metabolism
gut microbiota
experimental study
